| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOOK3 |
| Uniprot No | Q86VS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718aa |
| 氨基酸序列 | MFSVESLERA ELCESLLTWI QTFNVDAPCQ TVEDLTNGVV MAQVLQKIDP AYFDENWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV MTAIQELMSK ESPVSAGNDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH ELDMQVAALQ EEKSSLLAEN QVLMERLNQS DSIEDPNSPA GRRHLQLQTQ LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN TVSLEEELRK ANAARSQLET YKRQVVELQN RLSEESKKAD KLDFEYKRLK EKVDSLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEGSDNEKIA LLQSLLDDAN LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LKIEELQEAL RKKEEEMKQM EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE YEKTKSQREM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ ATSSRRSYPG HVQPATAR |
| 分子量 | 109.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOOK3蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*HOOK3 links dynactin to microtubule motors in cytoplasmic dynein-mediated transport*
**作者**：Schroeder, C.M., et al.
**摘要内容**：通过重组人HOOK3蛋白的体外实验，揭示了其作为连接动力蛋白复合体(dynein-dynactin)与微管马达的适配器功能，证明HOOK3在细胞质运输中协调囊泡与微管网络的锚定作用。
2. **文献名称**：*Structural characterization of the microtubule-binding domain of human HOOK3 protein*
**作者**：Guo, F., et al.
**摘要内容**：研究利用重组表达的HOOK3微管结合结构域，结合X射线晶体学分析其三维结构，阐明其与微管结合的分子机制，为HOOK3在细胞内运输中的作用提供结构依据。
3. **文献名称**：*Recombinant HOOK3 regulates endosomal trafficking via Rab5 interaction in HeLa cells*
**作者**：Yamashita, M., et al.
**摘要内容**：通过重组HOOK3蛋白的过表达实验，发现其与Rab5 GTPase的直接结合可调控早期内体运输，并影响EGFR等受体的降解过程，提示HOOK3在膜运输中的关键作用。
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**备注**：上述文献为示例，实际引用需根据具体研究核实。推荐通过PubMed或Google Scholar等平台，使用关键词“recombinant HOOK3 protein”、“human HOOK3 expression”等检索最新或高被引研究。
Recombinant human HOOK3 protein is a genetically engineered version of the HOOK3 protein, designed for experimental and therapeutic research. HOOK3 belongs to the Hook family of cytosolic proteins, which are evolutionarily conserved adaptors involved in intracellular vesicle trafficking and organelle positioning. Specifically, HOOK3 localizes to microtubules and facilitates the transport of endosomes, lysosomes, and other organelles by linking cargo to the dynein-dynactin motor complex. Its structure includes an N-terminal microtubule-binding domain, a central coiled-coil region for dimerization, and a C-terminal domain that interacts with cargo-specific proteins or complexes.
HOOK3 plays a critical role in cellular processes such as Golgi organization, autophagy, and receptor recycling. Dysregulation of HOOK3 has been implicated in diseases, including cancers (e.g., prostate, breast) and neurological disorders, due to its impact on vesicular transport and signaling pathways. Recombinant HOOK3 is typically produced in bacterial or mammalian expression systems, enabling studies on its molecular interactions, structural dynamics, and functional mechanisms.
Researchers utilize recombinant HOOK3 to investigate its role in cargo-specific trafficking, screen for small-molecule modulators, or develop diagnostic tools. Its application extends to disease modeling and elucidating pathways disrupted in HOOK3-associated pathologies. This protein’s versatility makes it a valuable tool for advancing both basic cell biology and translational research.
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