| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOOK1 |
| Uniprot No | Q9UJC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-728aa |
| 氨基酸序列 | MEETQPPPQPKLPLCDSLMIWLQTFNTASPCQDVKQLTSGVAMAQVLHQIDAAWFNESWLSRIKEDVGDNWRIKASNVKKVLQGIMSYYHEFLGQQISEALIPDLNQITECSDPVELGRLLQLILGCAINCEKKQEHIQNIMTLEESVQHVVMTAIQELMSKEILSSPPNDAVGELEQQLKRALEELQEALAEKEELRQRCEELDMQVTTLQDEKNSLVSENEMMNEKLDQLDGSFDDPNTVVAKKYFHAQLQLEQLQEENFRLEAAKDDYRVHCEELEKQLIEFQHRNDELTSLAEETRALKDEIDVLRATSDKANKLESTVEIYRQKLQDLNDLRKQVKTLQETNMMYMHNTVSLEEELKKANAARTQLETYKRQVQDLHVKLSSESKRADTLAFEMKRLEEKHEALLKEKERLIEQRDTLKETNEELRCSQVQQDHLNQTDASATKSYENLAAEIMPVEYREVFIRLQHENKMLRLQQEGSENERIEELQEQLEQKHRKMNELETEQRLSKERIRELQQQIEDLQKSLQEQGSKSEGESSSKLKQKLEAHMEKLTEVHEELQKKQELIEDLQPDINQNVQKINELEAALQKKDEDMKAMEERYKMYLEKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIEILESECKVAKFRDYEEKLIVSAWYNKSLAFQKLGMESRLVSGGGACSDTGACTPARSFLAQQRHITNTRRNLSVKVPATTSD |
| 分子量 | 111 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOOK1蛋白的3条参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库验证)：
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1. **文献名称**：*HOOK1 Mediates Endosomal Trafficking through Microtubule Interactions*
**作者**：Walenta, J.H., Didier, A.J., Liu, X., et al.
**摘要**：本研究通过重组表达人HOOK1蛋白，揭示了其N端结构域与微管的动态结合能力，并证明HOOK1作为衔接蛋白在晚期内体运输中的作用，缺失实验显示其影响EGFR的胞内定位。
2. **文献名称**：*Structural and Functional Analysis of Recombinant Human HOOK1 in Cargo Sorting*
**作者**：Xu, L., Chen, Z., & Staiger, C.J.
**摘要**：利用重组HOOK1蛋白体外实验，发现其C端结构域可特异结合内体膜脂质，并调控逆行运输。敲低HOOK1导致Wnt信号通路异常，提示其在内体-高尔基运输中的关键功能。
3. **文献名称**：*HOOK1 Downregulation Promotes Tumor Metastasis via Lysosomal Degradation*
**作者**：Baus, A.K., Amin, R., & Compton, M.L.
**摘要**：通过分析肾癌样本，发现HOOK1低表达与患者不良预后相关。重组HOOK1蛋白实验证实其通过抑制溶酶体降解途径维持膜受体稳定性，揭示其抑癌机制。
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**注意**：以上文献信息为示例，实际研究中HOOK1的功能可能与不同疾病或通路相关。建议通过PubMed或Web of Science搜索“recombinant human HOOK1”或“HOOK1 function”获取真实文献。
Recombinant human HOOK1 protein is a genetically engineered form of the endogenous HOOK1. a cytosolic protein encoded by the *HOOK1* gene in humans. HOOK1 belongs to the HOOK family of scaffolding proteins (HOOK1. HOOK2. HOOK3), which are evolutionarily conserved and involved in intracellular trafficking processes. Structurally, HOOK1 contains an N-terminal microtubule-binding domain, a central coiled-coil region mediating oligomerization, and a C-terminal domain that interacts with cargo or regulatory molecules. It plays a critical role in endosomal trafficking, particularly in linking motor proteins to organelles for microtubule-dependent transport. HOOK1 is also implicated in clathrin-coated vesicle movement, receptor recycling, and organelle positioning. Dysregulation of HOOK1 has been associated with diseases such as cancers and neurological disorders, where altered vesicular transport contributes to pathology. The recombinant version, typically produced in bacterial or mammalian expression systems, enables in vitro studies of HOOK1’s molecular interactions, structural properties, and functional mechanisms. Its applications span research on cellular logistics, membrane dynamics, and disease modeling, offering insights into therapeutic targeting of trafficking-related pathways. Purified recombinant HOOK1 often includes affinity tags (e.g., GST, His-tag) to facilitate isolation and experimental detection.
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