| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HORMAD2 |
| Uniprot No | Q8N7B1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307aa |
| 氨基酸序列 | MATAQLSHCITIHKASKETVFPSQITNEHESLKMVKKLFATSISCITYLRGLFPESSYGERHLDDLSLKILREDKKCPGSLHIIRWIQGCFDALEKRYLRMAVLTLYTDPMGSEKVTEMYQFKFKYTKEGATMDFDSHSSSTSFESGTNNEDIKKASVLLIRKLYILMQDLEPLPNNVVLTMKLHYYNAVTPHDYQPLGFKEGVNSHFLLFDKEPINVQVGFVSTGFHSMKVKVMTEATKVIDLENNLFRENSTTEIAHQGLDCDEEEECNDHIQRMNFVCSQQSSECSRKKRKVSEPVKVFIPNRK |
| 分子量 | 61.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HORMAD2蛋白的3篇参考文献示例(注：文献为虚构，仅示例格式)：
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1. **标题**：*Expression and functional analysis of recombinant human HORMAD2 in meiotic recombination*
**作者**：García-Cao I, et al.
**摘要**：研究通过大肠杆菌系统成功表达重组人HORMAD2蛋白，并证明其通过调控减数分裂同源重组修复过程参与生殖细胞发育，为不育症机制研究提供依据。
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2. **标题**：*HORMAD2 interacts with ATR kinase: A study using purified recombinant protein*
**作者**：Brill S, et al.
**摘要**：利用昆虫细胞体系纯化重组HORMAD2蛋白，揭示其与ATR激酶的物理互作及在DNA损伤应答中的调控作用，提示其在癌症中的潜在功能。
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3. **标题**：*Structural characterization of HORMAD2 N-terminal domain via recombinant protein crystallization*
**作者**：Moriyama T, et al.
**摘要**：通过哺乳动物表达系统获得重组HORMAD2蛋白N端结构域并解析其晶体结构，阐明其介导染色体联会特异性结合的分子机制。
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实际文献需通过PubMed等数据库检索确认。若需真实文献，建议补充关键词如“recombinant HORMAD2”或“HORMAD2 protein purification”进一步筛选。
HORMAD2 (HORMA domain-containing protein 2) is a member of the conserved HORMA protein family, named after yeast Hop1. mammalian Mad2. and Arabidopsis Asy1. It plays a critical role in meiotic processes, particularly in mammalian germ cell development. In humans, HORMAD2 is predominantly expressed in the testis and ovary, where it localizes to meiotic chromosomes during prophase I. It is essential for synapsis, recombination, and checkpoint control during meiosis. Structurally, it contains a characteristic HORMA domain that facilitates conformational changes and interactions with partner proteins.
Studies show HORMAD2 participates in monitoring homology between paired chromosomes, promoting double-strand break repair, and ensuring crossover formation. It also collaborates with synaptonemal complex proteins (e.g., SYCP1/3) and DNA damage response factors to maintain genomic stability. Aberrant HORMAD2 expression or mutations are linked to infertility and meiotic defects.
Recombinant human HORMAD2 protein, typically produced in bacterial or mammalian expression systems, enables in vitro studies of its biochemical properties, interaction networks, and structural dynamics. Its recombinant form is instrumental in deciphering molecular mechanisms of meiosis, developing diagnostic biomarkers for gametogenesis disorders, and exploring potential therapeutic targets for fertility-related conditions. Recent research also investigates its ectopic expression in cancers, suggesting possible roles in tumorigenesis.
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