| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXA4 |
| Uniprot No | Q00056 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-320aa |
| 氨基酸序列 | MTMSSFLINS NYIEPKFPPF EEYAQHSGSG GADGGPGGGP GYQQPPAPPT QHLPLQQPQL PHAGGGREPT ASYYAPRTAR EPAYPAAALY PAHGAADTAY PYGYRGGASP GRPPQPEQPP AQAKGPAHGL HASHVLQPQL PPPLQPRAVP PAAPRRCEAA PATPGVPAGG SAPACPLLLA DKSPLGLKGK EPVVYPWMKK IHVSAVNPSY NGGEPKRSRT AYTRQQVLEL EKEFHFNRYL TRRRRIEIAH TLCLSERQVK IWFQNRRMKW KKDHKLPNTK MRSSNSASAS AGPPGKAQTQ SPHLHPHPHP STSTPVPSSI |
| 分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and Expression of Human HOXA4 Protein in E. coli" by Zhang et al.**
- 该研究描述了人HOXA4基因的克隆策略及在大肠杆菌中的重组表达，优化了纯化方法并验证了蛋白的DNA结合活性。
2. **"HOXA4 Regulates Hematopoietic Stem Cell Differentiation" by Smith et al.**
- 通过体外实验表明，重组HOXA4蛋白参与调控造血干细胞分化的分子机制，并影响特定靶基因(如MEIS1)的转录激活。
3. **"Structural Analysis of HOXA4-DNA Interaction via Electrophoretic Mobility Shift Assay" by Lee and Kim**
- 利用重组HOXA4蛋白进行电泳迁移实验，揭示其与特定DNA序列的结合特性及关键结构域(如同源结构域)的功能重要性。
4. **"HOXA4 Overexpression Promotes Tumor Invasion in Gastric Cancer Models" by Chen et al.**
- 研究发现，重组HOXA4蛋白在胃癌细胞中异常高表达，可通过激活EMT通路(如调控SNAIL1)促进肿瘤细胞迁移和侵袭。
注：以上为模拟生成的参考文献，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
HOXA4. a member of the HOX family of transcription factors, plays critical roles in embryonic development and cellular differentiation. HOX genes are evolutionarily conserved and essential for establishing body segmentation patterns, particularly in the anterior-posterior axis. Specifically, HOXA4 is involved in the development of the vertebrate skeleton, nervous system, and organs such as the lungs and thymus. Its expression is tightly regulated during embryogenesis, influencing cell fate decisions and tissue morphogenesis.
Recombinant human HOXA4 protein is engineered for research applications, enabling studies on its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains the conserved homeodomain responsible for DNA binding and transcriptional regulation. Researchers use it to investigate HOXA4’s interaction with target genes, co-factors, or signaling pathways, including Wnt and TGF-β, which are implicated in developmental disorders and cancers. Dysregulation of HOXA4 has been linked to leukemia, lung diseases, and congenital malformations. Its recombinant form also aids in drug discovery, screening for molecules that modulate its activity. Despite progress, HOXA4’s context-dependent roles in tissue homeostasis and disease require further exploration, particularly its dual functions as an oncogene or tumor suppressor in different cancers.
×
