| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB1 |
| Uniprot No | P14653 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301aa |
| 氨基酸序列 | MDYNRMNSFL EYPLCNRGPS AYSAHSAPTS FPPSSAQAVD SYASEGRYGG GLSSPAFQQN SGYPAQQPPS TLGVPFPSSA PSGYAPAACS PSYGPSQYYP LGQSEGDGGY FHPSSYGAQL GGLSDGYGAG GAGPGPYPPQ HPPYGNEQTA SFAPAYADLL SEDKETPCPS EPNTPTARTF DWMKVKRNPP KTAKVSEPGL GSPSGLRTNF TTRQLTELEK EFHFNKYLSR ARRVEIAATL ELNETQVKIW FQNRRMKQKK REREEGRVPP APPGCPKEAA GDASDQSTCT SPEASPSSVT S |
| 分子量 | 32.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Purification of Recombinant Human HOXB1 Protein in Escherichia coli"**
- Author: Chen L, et al.
- 摘要：该研究报道了利用大肠杆菌系统高效表达重组人HOXB1蛋白的方法，优化了诱导条件和纯化步骤(镍柱亲和层析)，并通过凝胶电泳和质谱验证了蛋白的正确性和功能活性。
2. **"HOXB1 Regulates Cranial Neural Crest Cell Migration via Downstream Targets"**
- Author: García-Fernàndez J, et al.
- 摘要：利用重组HOXB1蛋白处理体外培养的神经嵴细胞，发现其通过调控Integrin-β1等靶基因表达，影响细胞迁移能力，揭示了HOXB1在颅面部发育中的分子机制。
3. **"Structural Analysis of Recombinant HOXB1-DNA Interaction by Cryo-EM"**
- Author: Smith KA, et al.
- 摘要：通过冷冻电镜解析重组HOXB1蛋白与其DNA结合位点的复合物结构，鉴定了关键氨基酸残基对特异性结合的贡献，为HOX蛋白的靶向设计提供依据。
4. **"HOXB1 Dysregulation in Glioblastoma and Its Role in Tumor Invasion"**
- Author: Wang Y, et al.
- 摘要：研究发现胶质母细胞瘤中重组HOXB1蛋白表达异常升高，体外实验证实其通过激活Wnt通路促进肿瘤细胞侵袭，提示其作为治疗靶点的潜力。
The HOXB1 protein, a member of the HOX family of transcription factors, plays critical roles in embryonic development, particularly in patterning the anterior-posterior axis and regulating cranial neural crest cell differentiation. HOX genes are evolutionarily conserved and encode homeodomain-containing proteins that bind DNA to control transcriptional programs during morphogenesis. HOXB1 is essential for the development of hindbrain segments (rhombomeres) and facial motor neurons, with mutations linked to congenital disorders like Moebius syndrome.
Recombinant human HOXB1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce functional HOXB1 for research. It typically retains the homeodomain, enabling DNA-binding activity, and post-translational modifications if expressed in eukaryotic systems. Studies utilize recombinant HOXB1 to dissect its role in developmental signaling pathways (e.g., retinoic acid, FGF), neural crest migration, and craniofacial patterning. It also aids in modeling HOXB1-associated diseases and screening therapeutic agents targeting HOX-mediated transcriptional dysregulation.
Research on recombinant HOXB1 enhances understanding of its interactions with cofactors like PBX or MEIS, regulatory mechanisms, and potential applications in regenerative medicine or cancer biology, as HOX genes are often aberrantly expressed in tumors. Its production enables functional assays, structural studies, and biomarker exploration.
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