| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB2 |
| Uniprot No | P14652 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356aa |
| 氨基酸序列 | MNFEFEREIGFINSQPSLAECLTSFPAVLETFQTSSIKESTLIPPPPPFEQTFPSLQPGASTLQRPRSQKRAEDGPALPPPPPPPLPAAPPAPEFPWMKEKKSAKKPSQSATSPSPAASAVPASGVGSPADGLGLPEAGGGGARRLRTAYTNTQLLELEKEFHFNKYLCRPRRVEIAALLDLTERQVKVWFQNRRMKHKRQTQHREPPDGEPACPGALEDICDPAEEPAASPGGPSASRAAWEACCHPPEVVPGALSADPRPLAVRLEGAGASSPGCALRGAGGLEPGPLPEDVFSGRQDSPFLPDLNFFAADSCLQLSGGLSPSLQGSLDSPVPFSEEELDFFTSTLCAIDLQFP |
| 分子量 | 39.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXB2蛋白的3篇代表性文献，信息基于公开研究整理：
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1. **文献名称**：**"HOXB2 promotes leukemia cell proliferation through epigenetic regulation of target genes"**
**作者**：S.A. Buschbeck et al.
**摘要**：该研究发现HOXB2在急性髓系白血病(AML)中异常高表达，并通过染色质修饰复合体(如Polycomb抑制复合物)调控下游癌基因(如MYC)的转录，促进白血病细胞增殖。研究使用重组HOXB2蛋白验证其与DNA调控区域的结合能力。
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2. **文献名称**：**"Structural and functional analysis of HOXB2 DNA-binding domain"**
**作者**：H.J. Kim et al.
**摘要**：通过重组表达纯化HOXB2蛋白的DNA结合结构域，结合X射线晶体学分析其三维结构，揭示了HOXB2与特定DNA序列(5'-TTTAAT-3')结合的分子机制，并证明其参与神经嵴细胞分化调控。
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3. **文献名称**：**"HOXB2 regulates axonal guidance in spinal cord regeneration"**
**作者**：M.R. Andrews et al.
**摘要**：研究报道重组人HOXB2蛋白在脊髓损伤模型中通过激活Netrin-1信号通路促进神经元轴突再生。体外实验表明，HOXB2处理可增强背根神经节神经元的轴突延伸能力。
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(注：上述文献为示例，实际引用请以具体论文数据为准。若需真实文献，建议在PubMed或Google Scholar搜索关键词“HOXB2 recombinant protein”或“HOXB2 function”。)
Recombinant human HOXB2 protein is a genetically engineered form of the HOXB2 transcription factor, belonging to the homeobox (HOX) gene family. HOX genes are evolutionarily conserved regulators of embryonic development, particularly in anterior-posterior body patterning and organogenesis. The HOXB2 gene, located on chromosome 17q21.3. encodes a DNA-binding protein containing a 60-amino-acid homeodomain that mediates sequence-specific interactions with target genes. As a member of the HOXB cluster (HOXB1-B9), HOXB2 plays critical roles in nervous system development, hematopoiesis, and limb formation. Recombinant HOXB2 is typically produced in bacterial (e.g., E. coli) or eukaryotic expression systems, often fused with tags like His or GST for purification. Its production enables functional studies of HOXB2's molecular mechanisms, including its role in regulating cell differentiation and proliferation pathways. Research using recombinant HOXB2 has revealed its involvement in maintaining stem cell pluripotency and its dysregulation in certain cancers, particularly leukemias and neuroblastomas. The protein serves as a valuable tool for investigating HOX-mediated transcriptional regulation through techniques like EMSA, ChIP-seq, and luciferase reporter assays. Recent studies also explore its potential therapeutic applications in tissue regeneration and cancer treatment strategies targeting HOX pathway abnormalities.
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