| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB5 |
| Uniprot No | P09067 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-269aa |
| 氨基酸序列 | MSSYFVNSFSGRYPNGPDYQLLNYGSGSSLSGSYRDPAAMHTGSYGYNYNGMDLSVNRSSASSSHFGAVGESSRAFPAPAQEPRFRQAASSCSLSSPESLPCTNGDSHGAKPSASSPSDQATSASSSANFTEIDEASASSEPEEAASQLSSPSLARAQPEPMATSTAAPEGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQP |
| 分子量 | 55.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXB5蛋白的3篇示例参考文献(注：文献为假设性示例，实际引用请通过学术数据库核实)：
1. **文献名称**：*HOXB5 promotes lung adenocarcinoma progression via transcriptional activation of oncogenic signaling*
**作者**：Li, X. et al.
**摘要**：该研究通过重组人HOXB5蛋白体外处理肺癌细胞，发现其通过激活Wnt/β-catenin信号通路促进肿瘤细胞的增殖和转移。实验证实HOXB5直接结合下游靶基因启动子区域。
2. **文献名称**：*Recombinant HOXB5 inhibits TGF-β-induced epithelial-mesenchymal transition in colorectal cancer*
**作者**：Zhang, Y. et al.
**摘要**：研究利用大肠杆菌表达系统制备重组人HOXB5蛋白，发现其可抑制TGF-β诱导的结直肠癌细胞上皮间质转化(EMT)，并通过下调Slug蛋白延缓肿瘤侵袭。
3. **文献名称**：*HOXB5 regulates self-renewal of hematopoietic stem cells through chromatin remodeling*
**作者**：Wang, L. et al.
**摘要**：通过重组HOXB5蛋白处理造血干细胞，研究发现其通过招募组蛋白修饰复合物维持干细胞干性，为白血病靶向治疗提供了潜在分子机制。
**注意事项**：
上述文献为简化示例，实际研究中建议通过PubMed、Web of Science等平台检索关键词“recombinant human HOXB5 protein”或“HOXB5 protein function”获取最新文献，并关注其在发育调控、癌症或再生医学领域的应用机制研究。
Homeobox B5 (HOXB5) is a member of the HOX family of transcription factors, which play critical roles in embryonic development, particularly in establishing anterior-posterior body patterning. HOX proteins contain a conserved 60-amino acid DNA-binding domain (homeodomain) that regulates the expression of target genes involved in cell differentiation, proliferation, and tissue identity. In humans, HOXB5 is encoded by the HOXB gene cluster on chromosome 17 and is primarily expressed during embryogenesis, where it contributes to the development of multiple organs, including the lungs, gut, and hematopoietic system.
In adult tissues, HOXB5 maintains roles in cellular homeostasis and regeneration. Studies suggest its involvement in stem cell regulation, epithelial-mesenchymal interactions, and wound healing. Dysregulation of HOXB5 has been implicated in pathologies such as cancers (e.g., leukemia, breast, and colorectal cancers), where it may act as an oncogene or tumor suppressor depending on context.
Recombinant human HOXB5 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for research. It serves as a tool to investigate HOXB5’s molecular mechanisms, interaction partners, and therapeutic potential. Applications include studying developmental biology, cancer progression, and regenerative medicine strategies. Its production enables *in vitro* assays, structural studies, and preclinical testing of HOXB5-targeted therapies.
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