| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB3 |
| Uniprot No | P14651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MQKATYYDNAAAALFGGYSSYPGSNGFGFDVPPQPPFQAATHLEGDYQRSACSLQSLGNAAPHAKSKELNGSCMRPGLAPEPLSAPPGSPPPSAAPTSATSNSSNGGGPSKSGPPKCGPGTNSTLTKQIFPWMKESRQTSKLKNNSPGTAEGCGGGGGGGGGGGSGGSGGGGGGGGGGDKSPPGSAASKRARTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLSERQIKIWFQNRRMKYKKDQKAKGLASSSGGPSPAGSPPQPMQSTAGFMNALHSMTPSYESPSPPAFGKAHQNAYALPSNYQPPLKGCGAPQKYPPTPAPEYEPHVLQANGGAYGTPTMQGSPVYVGGGGYADPLPPPAGPSLYGLNHLSHHPSGNLDYNGAPPMAPSQHHGPCEPHPTYTDLSSHHAPPPQGRIQEAPKLTHL |
| 分子量 | 73.81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人HOXB3蛋白的虚构参考文献示例(仅供参考)：
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**文献名称**：重组人HOXB3蛋白的表达纯化及功能验证
**作者**：Smith J. 等(2020)
**摘要**：本研究通过大肠杆菌系统高效表达了重组人HOXB3蛋白，优化了纯化工艺并验证其与DNA结合活性。实验表明，该蛋白可特异性识别靶基因启动子区域，为后续功能研究提供工具。
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**文献名称**：HOXB3蛋白在急性髓系白血病中的调控机制
**作者**：Chen L. 等(2018)
**摘要**：作者利用重组HOXB3蛋白处理白血病细胞，发现其通过激活Wnt/β-catenin通路促进细胞增殖，且在患者样本中HOXB3高表达与不良预后相关。
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**文献名称**：HOXB3蛋白结构解析及其与染色质互作研究
**作者**：Wang H. 等(2022)
**摘要**：通过X射线晶体学解析了重组HOXB3蛋白的DNA结合结构域三维结构，揭示了关键氨基酸残基在靶基因调控中的作用，为设计靶向抑制剂奠定基础。
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**文献名称**：重组HOXB3诱导神经干细胞分化的体外实验
**作者**：Kim S. 等(2019)
**摘要**：研究发现，外源性重组HOXB3蛋白可激活Notch信号通路，显著促进神经干细胞向神经元分化，为神经再生治疗提供潜在策略。
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注：以上内容为模拟生成，实际文献需通过学术数据库检索获取。
HOXB3. a member of the HOX gene family, encodes a transcription factor critical in embryonic development, particularly in establishing anterior-posterior body axis patterning and regulating cellular differentiation. HOX proteins bind DNA via homeodomains to control gene networks involved in morphogenesis, tissue identity, and cell proliferation. Dysregulation of HOXB3 has been linked to cancers, including leukemia and solid tumors, where it may act as an oncogene or tumor suppressor depending on context. For instance, HOXB3 overexpression promotes angiogenesis and metastasis in certain cancers, while its downregulation correlates with poor prognosis in others.
Recombinant human HOXB3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified protein for research. It typically includes tags (e.g., His-tag) for simplified purification and detection. This recombinant tool enables studies on HOXB3’s molecular mechanisms—such as its role in hematopoiesis, neural development, or epithelial-mesenchymal transition—and its interplay with signaling pathways like Wnt or TGF-β. Researchers also utilize it to screen inhibitors targeting HOXB3-driven oncogenic pathways or to explore regenerative applications. Despite progress, HOXB3’s context-dependent functions in malignancy and tissue homeostasis remain incompletely understood, necessitating further structural and functional analyses using recombinant forms.
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