| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB8 |
| Uniprot No | P17481 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-243aa |
| 氨基酸序列 | MSSYFVNSLFSKYKTGESLRPNYYDCGFAQDLGGRPTVVYGPSSGGSFQHPSQIQEFYHGPSSLSTAPYQQNPCAVACHGDPDNFYGYDPLQRQSLFGAQDPDLVQYADCKLAAASGLGEEAEGSEQSPSPTQLFPWMRPQAAAGRRRGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKFHSSKCEQEELEKQKLERAPEAADEGDAQKGDKK |
| 分子量 | 54.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXB8蛋白的3篇文献概览(基于公开研究主题总结，非具体文献复现)：
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1. **文献名称**：*HOXB8 mediates erythroleukemia cell proliferation via Wnt/β-catenin signaling*
**作者**：Argiropoulos B, et al.
**摘要**：探讨重组人HOXB8蛋白在白血病细胞系中的促增殖作用，发现其通过激活Wnt/β-catenin通路促进细胞周期进展，为HOXB8在髓系白血病中的致癌机制提供证据。
2. **文献名称**：*Recombinant HOXB8 enhances self-renewal of hematopoietic stem cells in vitro*
**作者**：Smith CE, et al.
**摘要**：研究重组HOXB8蛋白对造血干细胞的影响，证实其通过调控Notch信号通路增强干细胞的自我更新能力，提示其在再生医学中的潜在应用价值。
3. **文献名称**：*Structural characterization of recombinant human HOXB8 transcription factor*
**作者**：Kim D, et al.
**摘要**：利用大肠杆菌表达系统成功纯化重组HOXB8蛋白，通过X射线晶体学解析其三维结构，揭示其DNA结合域的关键氨基酸残基，为靶向药物设计提供结构基础。
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**注**：以上文献为示例性质，具体内容需参考真实发表的科研论文。建议通过PubMed或Web of Science以“recombinant human HOXB8”为关键词检索获取准确信息。
HOXB8. a member of the homeobox (HOX) gene family, encodes transcription factors critical for embryonic development, tissue patterning, and cellular differentiation. HOX proteins regulate gene expression by binding DNA via their conserved 60-amino acid homeodomain. Human HOXB8 specifically plays roles in hematopoiesis, nervous system development, and cell proliferation. It has dual implications in cancer, acting as an oncogene in leukemia, glioblastoma, and colorectal cancer, while showing tumor-suppressive effects in breast and ovarian cancers. This context-dependent behavior highlights its complex regulatory networks.
Recombinant human HOXB8 protein is engineered using expression systems (e.g., E. coli or mammalian cells) for functional studies. Its production enables in vitro exploration of DNA-protein interactions, signaling pathways (e.g., Wnt/β-catenin), and epigenetic mechanisms. Researchers utilize purified HOXB8 to investigate its role in stem cell maintenance, differentiation, and malignancy. Structural studies focus on its homeodomain and protein-binding motifs to design inhibitors. Challenges include its intrinsic disorder regions and post-translational modifications affecting stability. As a research tool, recombinant HOXB8 aids in decoding developmental biology and cancer pathology, though clinical applications remain speculative.
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