| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXC12 |
| Uniprot No | P31275 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MGEHNLLNPG FVGPLVNIHT GDTFYFPNFR ASGAQLPGLP SLSYPRRDNV CSLSWPSAEP CNGYPQPYLG SPVSLNPPFG RTCELARVED GKGYYREPCA EGGGGGLKRE ERGRDPGAGP GAALLPLEPS GPPALGFKYD YAAGGGGGDG GGGAGPPHDP PSCQSLESDS SSSLLNEGNK GAGAGDPGSL VSPLNPGGGL SASGAPWYPI NSRSRKKRKP YSKLQLAELE GEFLVNEFIT RQRRRELSDR LNLSDQQVKI WFQNRRMKKK RLLLREQALS FF |
| 分子量 | 30.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXC12蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "Expression and Purification of Recombinant Human HOXC12 Protein in E. coli"
**作者**: Zhang Y, et al.
**摘要**: 报道了通过大肠杆菌表达系统成功克隆并纯化重组人HOXC12蛋白，验证其核定位特性及DNA结合能力，为后续功能研究提供工具。
2. **文献名称**: "HOXC12 Promotes Tumor Metastasis via Transcriptional Regulation of EMT Markers"
**作者**: Li H, Wang X, Chen J
**摘要**: 研究发现重组HOXC12蛋白在癌细胞中过表达可上调上皮-间质转化(EMT)相关基因(如Snail、Vimentin)，促进肿瘤侵袭转移。
3. **文献名称**: "Structural Analysis of HOXC12-DNA Interaction by Cryo-EM"
**作者**: Gupta R, et al.
**摘要**: 利用冷冻电镜解析重组HOXC12蛋白与靶DNA的复合物结构，揭示其同源结构域特异性结合的分子机制，为靶向药物设计提供依据。
4. **文献名称**: "HOXC12 in Skeletal Development: Insights from Knockout Mouse Models"
**作者**: Müller S, et al.
**摘要**: 通过重组HOXC12蛋白回补实验，证实其在胚胎肢体发育中调控成骨细胞分化的功能，缺失导致小鼠骨骼形态异常。
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**提示**：实际研究中建议通过以下方式查找文献：
- **数据库**：PubMed、Web of Science、Google Scholar
- **关键词**："recombinant HOXC12 protein"、"HOXC12 function"、"HOXC12 gene regulation"
- **筛选建议**：优先选择近5年发表、高影响因子期刊及权威团队的研究。
**Background of Recombinant Human HOXC12 Protein**
The HOXC12 protein, a member of the homeobox (HOX) transcription factor family, plays crucial roles in embryonic development, particularly in anterior-posterior body patterning and tissue differentiation. HOX genes encode conserved DNA-binding proteins that regulate transcriptional networks controlling cellular identity, proliferation, and morphogenesis. HOXC12. located on chromosome 12 within the HOXC cluster, is expressed in specific spatial and temporal patterns during embryogenesis. Dysregulation of HOXC12 has been implicated in developmental disorders, certain cancers (e.g., leukemia, solid tumors), and abnormal cell differentiation.
Recombinant human HOXC12 protein is engineered for in vitro studies to explore its biological functions and mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the protein is typically purified using affinity chromatography, often with tags like His or GST for detection and isolation. Its recombinant form enables investigation of DNA-binding properties, interactions with cofactors (e.g., PBX or MEIS proteins), and roles in gene regulatory pathways. Researchers utilize it to dissect HOXC12's involvement in developmental processes, disease pathogenesis, and potential therapeutic targeting. Quality control includes assays for purity, stability, and functional activity (e.g., electrophoretic mobility shift assays). Studies on recombinant HOXC12 contribute to understanding HOX protein dysfunction in human diseases and developmental biology.
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