| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXC9 |
| Uniprot No | P31274 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-260aa |
| 氨基酸序列 | MSATGPISNYYVDSLISHDNEDLLASRFPATGAHPAAARPSGLVPDCSDFPSCSFAPKPAVFSTSWAPVPSQSSVVYHPYGPQPHLGADTRYMRTWLEPLSGAVSFPSFPAGGRHYALKPDAYPGRRADCGPGEGRSYPDYMYGSPGELRDRAPQTLPSPEADALAGSKHKEEKADLDPSNPVANWIHARSTRKKRCPYTKYQTLELEKEFLFNMYLTRDRRYEVARVLNLTERQVKIWFQNRRMKMKKMNKEKTDKEQS |
| 分子量 | 54.34 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HOXC9蛋白的3-4篇代表性文献(部分为假设或简化的示例，供参考)：
1. **《HOXC9 regulates self-renewal and chemotherapy resistance in glioblastoma stem cells》**
- 作者：Smith A et al.
- 摘要：研究揭示HOXC9通过调控Wnt/β-catenin信号通路维持胶质母细胞瘤干细胞的自我更新能力，抑制重组HOXC9蛋白活性可降低肿瘤干细胞增殖并增强化疗敏感性。
2. **《HOXC9-mediated epigenetic reprogramming drives neuroblastoma chemoresistance》**
- 作者：Li Y et al.
- 摘要：发现HOXC9在成神经细胞瘤中高表达，重组HOXC9蛋白促进DNA甲基化酶DNMT3B的募集，导致抑癌基因沉默，进而介导化疗耐药。
3. **《Recombinant HOXC9 Protein Stabilizes SOX2 Transcription Factor to Maintain Pluripotency in Embryonic Stem Cells》**
- 作者：Chen L et al.
- 摘要：证明重组HOXC9蛋白通过直接结合SOX2的启动子区域，增强其转录活性，从而维持胚胎干细胞的未分化状态和多能性。
4. **《Structural Basis of HOXC9-DNA Interaction: Insights from Crystallography and Functional Assays》**
- 作者：Wang X et al.
- 摘要：解析了重组HOXC9蛋白的晶体结构，揭示了其同源域关键氨基酸与DNA结合的分子机制，为设计靶向HOXC9的小分子药物提供结构基础。
**注**：以上文献标题和内容为知识库示例，具体内容需结合真实发表的论文调整。建议通过PubMed或Google Scholar使用关键词“recombinant HOXC9 protein”或“HOXC9 functional study”查询最新研究。
Recombinant human HOXC9 protein is a genetically engineered form of the HOXC9 transcription factor, a member of the Homeobox (Hox) gene family. Hox genes play critical roles in embryonic development, particularly in establishing the body plan along the anterior-posterior axis. HOXC9. located on chromosome 12. is implicated in regulating cellular differentiation, tissue patterning, and organogenesis, with specific roles in neural, skeletal, and limb development. Dysregulation of HOXC9 has been associated with cancers, where it may act as either an oncogene or tumor suppressor depending on cellular context. For instance, reduced HOXC9 expression correlates with aggressive phenotypes in certain tumors, while overexpression may inhibit metastasis.
The recombinant protein is typically produced in prokaryotic (e.g., E. coli) or eukaryotic expression systems, enabling studies of its structure, DNA-binding properties, and interactions with cofactors. Researchers utilize recombinant HOXC9 to investigate its regulatory mechanisms, including modulation of target genes involved in cell proliferation, apoptosis, and epithelial-mesenchymal transition. Its purified form also facilitates high-throughput screening for potential therapeutics targeting Hox-related diseases. As a tool in developmental biology and oncology, recombinant HOXC9 provides insights into both normal morphogenesis and pathological processes, bridging basic research with clinical applications.
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