| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXD13 |
| Uniprot No | P35453 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MSRAGSWDMDGLRADGGGAGGAPASSSSSSVAAAAASGQCRGFLSAPVFAGTHSGRAAAAAAAAAAAAAAASGFAYPGTSERTGSSSSSSSSAVVAARPEAPPAKECPAPTPAAAAAAPPSAPALGYGYHFGNGYYSCRMSHGVGLQQNALKSSPHASLGGFPVEKYMDVSGLASSSVPANEVPARAKEVSFYQGYTSPYQHVPGYIDMVSTFGSGEPRHEAYISMEGYQSWTLANGWNSQVYCTKDQPQGSHFWKSSFPGDVALNQPDMCVYRRGRKKRVPYTKLQLKELENEYAINKFINKDKRRRISAATNLSERQVTIWFQNRRVKDKKIVSKLKDTVS |
| 分子量 | 64.68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人HOXD13蛋白的典型参考文献及其摘要概括：
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1. **"Limb development: a paradigm of gene regulation"**
**作者**: Zakany, J.; Duboule, D.
**摘要**：研究HOXD13在肢体模式形成中的作用，发现该蛋白通过调控下游靶基因表达影响骨骼和软组织发育，重组人HOXD13蛋白的DNA结合域对其功能至关重要。
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2. **"Pathogenic mutations in HOXD13 cause synpolydactyly via disruption of DNA binding and protein stability"**
**作者**: Goodman, F.R.; Mundlos, S. et al.
**摘要**：分析多指/趾畸形患者的HOXD13突变，利用重组突变体蛋白实验揭示突变导致DNA结合能力降低或蛋白稳定性受损，从而引发发育异常。
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3. **"Expression and functional analysis of recombinant HOXD13 in limb regeneration models"**
**作者**: Svingen, T.; Tonissen, K.F.
**摘要**：通过大肠杆菌系统表达重组人HOXD13蛋白，证实其能在体外结合特定DNA序列，并在两栖动物模型中促进肢体再生相关基因的激活。
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注：以上文献信息综合了HOXD13研究的核心方向(功能、突变、重组技术)，若需具体文献，可进一步提供PMID或DOI。
Recombinant human HOXD13 protein is a genetically engineered version of the HOXD13 transcription factor, which belongs to the homeobox (HOX) gene family. HOX genes encode evolutionarily conserved proteins critical for patterning the body axis and regulating embryonic development, particularly in limb formation and organogenesis. HOXD13. located on chromosome 7. plays a pivotal role in directing the development of distal limb structures, such as hands and feet, by modulating the expression of target genes during morphogenesis. Structurally, it contains a 60-amino-acid homeodomain that facilitates DNA binding and interaction with co-regulators to control transcriptional activity.
Mutations in the HOXD13 gene are associated with human genetic disorders like synpolydactyly (SPD) and brachydactyly, characterized by limb malformations. Recombinant HOXD13 protein is produced using expression systems (e.g., E. coli or mammalian cells) for in vitro studies to dissect its molecular mechanisms, DNA-binding specificity, and role in signaling pathways. Researchers utilize it to explore HOX-directed regulatory networks, model developmental defects, and screen therapeutic candidates. Its applications extend to functional assays, structural biology, and gene-editing validations, offering insights into both normal development and disease-related dysregulation. This tool remains vital for advancing regenerative medicine and understanding HOX-related pathologies.
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