| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXD11 |
| Uniprot No | P31277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338aa |
| 氨基酸序列 | MNDFDECGQSAASMYLPGCAYYVAPSDFASKPSFLSQPSSCQMTFPYSSNLAPHVQPVREVAFRDYGLERAKWPYRGGGGGGSAGGGSSGGGPGGGGGGAGGYAPYYAAAAAAAAAAAAAEEAAMQRELLPPAGRRPDVLFKAPEPVCAAPGPPHGPAGAASNFYSAVGRNGILPQGFDQFYEAAPGPPFAGPQPPPPPAPPQPEGAADKGDPRTGAGGGGGSPCTKATPGSEPKGAAEGSGGDGEGPPGEAGAEKSSSAVAPQRSRKKRCPYTKYQIRELEREFFFNVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLNRDRLQYFTGNPLF |
| 分子量 | 37.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人HOXD11蛋白的模拟参考文献及核心内容概述(基于领域知识推测，非真实文献)：
---
**1. 文献名称**：HOXD11重组蛋白通过抑制Wnt信号通路调控骨肉瘤细胞迁移
**作者**：Chen L, et al.
**摘要**：研究通过原核表达系统制备重组人HOXD11蛋白，发现其能显著抑制骨肉瘤细胞的迁移能力。机制研究表明，HOXD11通过结合Wnt5a启动子区域，下调Wnt/β-catenin通路活性，提示其作为骨肉瘤潜在治疗靶点。
---
**2. 文献名称**：人HOXD11蛋白的结构解析及其与DNA结合的关键位点
**作者**：Gupta R, Smith J
**摘要**：利用X射线晶体学首次解析HOXD11同源结构域的三维结构，鉴定出第50位精氨酸为DNA结合关键位点。通过重组蛋白突变体实验证实该位点突变会完全丧失调控下游基因转录的功能。
---
**3. 文献名称**：重组HOXD11蛋白在斑马鱼肢体再生模型中的促分化作用
**作者**：Tanaka H, et al.
**摘要**：在原核系统中表达并纯化活性HOXD11蛋白，显微注射至斑马鱼断肢模型后，显著加速软骨细胞分化。RNA-seq分析显示其通过激活Sox9和Col2a1表达促进组织修复，为再生医学提供新策略。
---
注：以上为模拟摘要，实际研究请参考具体文献数据库(如PubMed)。
Recombinant human HOXD11 protein is a genetically engineered version of the HOXD11 transcription factor, which belongs to the homeobox (HOX) gene family. HOX genes encode evolutionarily conserved proteins critical for embryonic development, particularly in establishing body patterning, limb formation, and axial specification. HOXD11. located on chromosome 2. is part of the HOXD cluster (paralogs D9-D13) and plays a key role in vertebrate limb morphogenesis, influencing proximal-distal patterning and skeletal development in posterior limbs. It binds DNA through its homeodomain, regulating downstream genes involved in cellular differentiation and tissue organization.
Recombinant HOXD11 is typically produced in heterologous systems like E. coli or mammalian cells, enabling scalable purification for research. Its recombinant form facilitates studies on molecular mechanisms in developmental biology, including how HOX proteins orchestrate spatial identity during embryogenesis. Dysregulation of HOXD11 has been implicated in congenital limb malformations and certain cancers, making it a focus for understanding disease pathways. Researchers also utilize recombinant HOXD11 to explore potential regenerative therapies or tissue engineering applications by manipulating HOX-directed cellular programming. Current challenges include deciphering context-specific interactions with cofactors and epigenetic regulators, which dictate its functional outcomes in diverse biological contexts.
×
