| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTCP |
| Uniprot No | Q14973 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-349aa |
| 氨基酸序列 | MEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLGKVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYIYSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPLLIATSSLMPFIGFLLGYVLSALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMIYTAATTEETIPGALGNGTYKGEDCSPCTA |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTCP(钠牛磺胆酸共转运多肽)重组蛋白的3篇代表性文献摘要：
1. **文献名称**：*Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus*
**作者**：Yan H. et al.
**摘要**：该研究首次证实NTCP是乙型肝炎病毒(HBV)和丁型肝炎病毒(HDV)进入肝细胞的关键受体，通过重组人NTCP蛋白在体外细胞模型中验证了其介导病毒感染的机制，为抗病毒药物开发提供了靶点。
2. **文献名称**：*Cryo-EM structure of the human sodium taurocholate cotransporting polypeptide*
**作者**：Shi Y. et al.
**摘要**：本研究利用冷冻电镜技术解析了重组人NTCP蛋白的高分辨率三维结构，揭示了其底物(胆汁酸)结合域和跨膜转运机制，为理解NTCP的生理功能及设计靶向分子奠定结构基础。
3. **文献名称**：*NTCP-reconstituted in vitro models for studying hepatitis B virus infection and drug entry inhibition*
**作者**：Watashi K. et al.
**摘要**：作者构建了NTCP重组蛋白表达的体外肝细胞模型，证明其可用于模拟HBV/HDV感染过程，并筛选出环孢菌素衍生物等NTCP抑制剂，为抗病毒药物高通量筛选提供了平台。
(注：以上文献信息为示例性概括，实际引用请以具体论文内容为准。)
**Background of NTCP Recombinant Protein**
The sodium taurocholate co-transporting polypeptide (NTCP), encoded by the *SLC10A1* gene, is a hepatocyte-specific transporter critical for bile acid homeostasis. Primarily located on the basolateral membrane of liver cells, NTCP mediates the uptake of conjugated bile acids, such as taurocholate, from the bloodstream into hepatocytes via sodium-dependent co-transport. Beyond its physiological role, NTCP gained significant attention in virology when identified in 2012 as the functional receptor for hepatitis B virus (HBV) and hepatitis D virus (HDV) entry, revolutionizing research into HBV/HDV pathogenesis and therapeutic targeting.
Recombinant NTCP proteins are engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional NTCP for *in vitro* studies. These proteins enable detailed structural and functional analyses, including ligand-binding assays, transport kinetics, and interaction studies with viral particles or inhibitors. For instance, recombinant NTCP has been instrumental in screening antiviral compounds like Myrcludex B, a first-in-class entry inhibitor blocking HBV/HDV attachment.
Additionally, NTCP’s role in drug disposition—particularly its interactions with cholesterol-lowering agents and other therapeutics—has spurred interest in pharmacokinetic research. Recombinant NTCP models help predict drug-transporter interactions, minimizing off-target effects during drug development. Recent structural insights from cryo-EM studies of NTCP have further guided rational drug design, emphasizing its dual relevance in basic science and clinical applications.
Overall, NTCP recombinant proteins serve as indispensable tools for unraveling liver physiology, viral entry mechanisms, and drug discovery, bridging gaps between molecular biology and translational medicine.
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