| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ND1 |
| Uniprot No | P03886 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318aa |
| 氨基酸序列 | MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ND1重组蛋白的3篇代表性文献摘要整理：
1. **《Functional analysis of ND1 mutations in mitochondrial diseases through recombinant protein expression》**
(作者：Smith J, et al.)
摘要：研究通过在大肠杆菌中重组表达人源ND1蛋白，结合定点突变技术，揭示了ND1亚基关键氨基酸位点突变(如m.3460G>A)对线粒体复合物I组装及酶活性的影响，为线粒体疾病机制提供了分子层面的解释。
2. **《Crystallographic study of recombinant ND1 subunit reveals structural insights into proton translocation》**
(作者：Tanaka R, et al.)
摘要：利用昆虫细胞系统重组表达并纯化ND1蛋白，通过X射线晶体学解析其三维结构，阐明了ND1在复合物I质子传递通道中的构象变化，为开发靶向复合物I的药物提供了结构基础。
3. **《Recombinant ND1-based serodiagnosis for mitochondrial antibody detection in autoimmune hepatitis》**
(作者：Chen L, et al.)
摘要：通过原核表达系统制备重组ND1抗原，建立ELISA检测方法，验证了其在自身免疫性肝炎患者血清中抗线粒体抗体的高特异性诊断价值，优化了临床检测流程。
注：以上文献信息为模拟示例，实际研究中建议通过PubMed或Web of Science以“ND1 recombinant protein”“mitochondrial complex I ND1”等关键词检索最新文献。
**Background of ND1 Recombinant Protein**
ND1 (NADH dehydrogenase subunit 1) is a critical component of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the first enzyme in the electron transport chain responsible for oxidative phosphorylation. Encoded by mitochondrial DNA (mtDNA), ND1 plays a vital role in electron transfer during ATP synthesis, contributing to cellular energy production. Mutations in the ND1 gene are linked to mitochondrial disorders, such as Leber’s hereditary optic neuropathy (LHON) and Leigh syndrome, highlighting its importance in mitochondrial function.
Recombinant ND1 protein is engineered using genetic engineering techniques, where the ND1 gene is cloned into expression vectors (e.g., bacterial, yeast, or mammalian systems) to produce purified protein for research. This approach enables large-scale production of ND1. circumventing challenges in isolating it from native tissues due to low mitochondrial protein yields.
Studying recombinant ND1 aids in elucidating Complex I structure-function relationships, mutation impacts, and disease mechanisms. It is also used to develop therapeutic strategies, including drug screening for mitochondrial disorders or gene therapy. Additionally, recombinant ND1 serves as an antigen for antibody production in diagnostic assays.
However, producing functional ND1 remains challenging due to its hydrophobic nature and dependence on mitochondrial chaperones for proper folding. Advanced expression systems, such as cell-free or yeast mitochondrial platforms, are often employed to improve solubility and activity.
In summary, ND1 recombinant protein is a vital tool for advancing mitochondrial biology research, disease modeling, and therapeutic innovation, bridging gaps between genetic insights and clinical applications.
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