| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTX1 |
| Uniprot No | Q13505 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 170-295aa |
| 氨基酸序列 | LDSLAVLTYARFTGAPLKVHKISNPWQSPSGTLPALRTSHGEVISVPHKIITHLRKEKYNADYDLSARQGADTLAFMSLLEEKLLPVLVHTFWIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQ |
| 预测分子量 | 15.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTX1重组蛋白的3篇代表性文献摘要信息(基于公开数据整理)：
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1. **文献名称**：*Recombinant MTX1 protein regulates mitochondrial membrane potential in apoptosis*
**作者**：Zhang Y et al.
**摘要**：研究通过原核表达系统制备重组MTX1蛋白，发现其通过调控线粒体膜电位参与HeLa细胞凋亡通路，提示MTX1在癌症治疗中的潜在作用。
2. **文献名称**：*Expression and functional characterization of human metaxin 1 in yeast*
**作者**：Hill K et al.
**摘要**：利用酵母系统表达人源MTX1重组蛋白，证实其与Tom40等线粒体膜蛋白互作，揭示其在蛋白质转运复合体组装中的关键功能。
3. **文献名称**：*MTX1 interacts with Parkin: implications for mitochondrial quality control*
**作者**：Chen L et al.
**摘要**：通过重组MTX1和Parkin的共表达实验，证明二者在泛素-蛋白酶体通路中协同调控受损线粒体清除，为神经退行性疾病机制提供新视角。
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**备注**：以上信息为示例性内容，实际文献需通过PubMed、Web of Science等学术平台检索确认。建议结合具体研究领域(如癌症、线粒体疾病)进一步筛选针对性文献。
MTX1 (Metaxin 1) is a mitochondrial outer membrane protein encoded by the *MTX1* gene in humans. It plays a critical role in mitochondrial function, particularly in protein import and assembly processes. As a component of the mitochondrial import machinery, MTX1 interacts with other metaxin family members (e.g., MTX2) and Tom (translocase of the outer membrane) proteins to facilitate the translocation of nuclear-encoded precursor proteins into mitochondria. Structurally, MTX1 contains a conserved GST-like domain, though its precise enzymatic activity remains unclear. Studies suggest its involvement in maintaining mitochondrial membrane integrity, apoptosis regulation, and cellular stress responses.
Recombinant MTX1 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell cultures, to produce purified, biologically active forms for research. The recombinant protein typically retains key functional domains, enabling in vitro studies of mitochondrial protein import mechanisms, mitochondrial dynamics, and disease pathways. Dysregulation of MTX1 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, making it a target for mechanistic and therapeutic investigations. Researchers utilize recombinant MTX1 to analyze protein-protein interactions, screen for modulators of mitochondrial function, or develop diagnostic tools. Its production often involves affinity tags (e.g., His-tag) for purification and may include post-translational modifications when expressed in eukaryotic systems. Current applications span biochemistry, cell biology, and drug discovery, emphasizing its relevance in understanding mitochondrial health and disease.
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