| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSTO1 |
| Uniprot No | Q9BUK6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-570aa |
| 氨基酸序列 | MAGGAREVLT LQLGHFAGFV GAHWWNQQDA ALGRATDSKE PPGELCPDVL YRTGRTLHGQ ETYTPRLILM DLKGSLSSLK EEGGLYRDKQ LDAAIAWQGK LTTHKEELYP KNPYLQDFLS AEGVLSSDGV WRVKSIPNGK GSSPLPTATT PKPLIPTEAS IRVWSDFLRV HLHPRSICMI QKYNHDGEAG RLEAFGQGES VLKEPKYQEE LEDRLHFYVE ECDYLQGFQI LCDLHDGFSG VGAKAAELLQ DEYSGRGIIT WGLLPGPYHR GEAQRNIYRL LNTAFGLVHL TAHSSLVCPL SLGGSLGLRP EPPVSFPYLH YDATLPFHCS AILATALDTV TVPYRLCSSP VSMVHLADML SFCGKKVVTA GAIIPFPLAP GQSLPDSLMQ FGGATPWTPL SACGEPSGTR CFAQSVVLRG IDRACHTSQL TPGTPPPSAL HACTTGEEIL AQYLQQQQPG VMSSSHLLLT PCRVAPPYPH LFSSCSPPGM VLDGSPKGAA VESIPVFGAL CSSSSLHQTL EALARDLTKL DLRRWASFMD AGVEHDDVAE LLQELQSLAQ CYQGGDSLVD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MSTO1重组蛋白的假设参考文献示例(仅供学术写作参考，非真实文献)：
1. **标题**: *Structural Insights into the Mitochondrial Distribution and Morphology Protein MSTO1 through Recombinant Expression*
**作者**: Zhang, Y. et al.
**摘要**: 本研究通过在大肠杆菌中重组表达并纯化人源MSTO1蛋白，结合X射线晶体学解析其三维结构，揭示了MSTO1的二聚化界面及与线粒体膜互作的关键结构域，为理解其调控线粒体形态的分子机制提供依据。
2. **标题**: *MSTO1 Knockout and Rescue with Recombinant Protein in Cellular Disease Models*
**作者**: Patel, R. et al.
**摘要**: 利用CRISPR技术构建MSTO1缺陷型细胞系，发现线粒体片段化表型;通过添加重组MSTO1蛋白可部分恢复线粒体网络连续性，表明其在维持线粒体动力学中具有直接功能。
3. **标题**: *Optimization of Recombinant MSTO1 Production in Mammalian Expression Systems for Functional Studies*
**作者**: Kim, H. & Lee, S.
**摘要**: 系统比较哺乳动物表达系统(如HEK293)中MSTO1重组蛋白的产量与活性，优化纯化策略后获得高纯度蛋白，并验证其与已知互作蛋白(如MFN2)的结合能力。
4. **标题**: *MSTO1 Mutations Impair Mitochondrial Function and Are Rescued by Recombinant Protein Supplementation*
**作者**: García-Sánchez, A. et al.
**摘要**: 在遗传性神经肌肉疾病患者中发现MSTO1突变位点，体外实验表明突变导致蛋白稳定性下降;外源性重组野生型MSTO1可恢复细胞中线粒体嵴结构及ATP合成能力。
注：以上为模拟生成的参考文献，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。
**Background of MSTO1 Recombinant Protein**
MSTO1 (Misato Family Protein 1) is a mitochondrial protein implicated in regulating mitochondrial dynamics and cellular homeostasis. It is evolutionarily conserved and shares homology with fungal Misato proteins, which are involved in mitochondrial distribution and morphology. Human MSTO1 localizes to mitochondria and interacts with components of the mitochondrial fission and fusion machinery, influencing mitochondrial network organization. Dysregulation of MSTO1 has been linked to mitochondrial disorders, including cerebellar ataxia and myopathy, due to its role in maintaining mitochondrial integrity and function.
Recombinant MSTO1 protein is engineered for *in vitro* studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains critical for interactions with mitochondrial proteins like dynamin-related GTPases (e.g., DRP1) or fusion mediators (e.g., MFN1/2). Researchers utilize MSTO1 recombinant protein to investigate its impact on mitochondrial morphology, apoptosis, and energy metabolism. It also serves as an antigen for antibody development or a tool in drug screening for mitochondrial-related diseases.
Structural studies of recombinant MSTO1 highlight its N-terminal α-helical domains and disordered regions, which may facilitate dynamic protein interactions. Mutational analyses using recombinant variants help identify residues essential for mitochondrial localization or binding partners. Current research explores MSTO1’s dual roles in mitochondrial dynamics and potential crosstalk with cellular stress pathways, offering insights into therapeutic strategies for mitochondrial disorders.
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