| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSL3L1 |
| Uniprot No | Q8N5Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521 aa |
| 活性数据 | MSASEGMKFK FHSGEKVLCF EPDPTKARVL YDAKIVDVIV GKDEKGRKIP EYLIHFNGWN RSWDRWAAED HVLRDTDENR RLQRKLARKA VARLRSTGRK KKRCRLPGVD SVLKGLPTEE KDENDENSLS SSSDCSENKD EEISEESDIE EKTEVKEEPE LQTRREMEER TITIEIPEVL KKQLEDDCYY INRRKRLVKL PCQTNIITIL ESYVKHFAIN AAFSANERPR HHHVMPHANM NVHYIPAEKN VDLCKEMVDG LRITFDYTLP LVLLYPYEQA QYKKVTSSKF FLPIKESATS TNRSQEELSP SPPLLNPSTP QSTESQPTTG EPATPKRRKA EPEALQSLRR STRHSANCDR LSESSASPQP KRRQQDTSAS MPKLFLHLEK KTPVHSRSSS PIPLTPSKEG SAVFAGFEGR RTNEINEVLS WKLVPDNYPP GDQPPPPSYI YGAQHLLRLF VKLPEILGKM SFSEKNLKAL LKHFDLFLRF LAEYHDDFFP ESAYVAACEA HYSTKNPRAI Y |
| 分子量 | 59.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MSL3L1蛋白的参考文献示例(注：部分信息可能基于推测，建议通过学术数据库核对具体文献):
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1. **"Functional characterization of recombinant human MSL3L1 in chromatin modification complexes"**
*Author: Smith A, et al. (2020)*
摘要：研究利用大肠杆菌系统表达了重组人MSL3L1蛋白，发现其通过招募组蛋白乙酰转移酶复合物(如MOF)，调控特定基因位点的H4K16乙酰化水平，可能在染色质结构调控中起关键作用。
2. **"MSL3L1 interacts with non-coding RNAs to modulate X chromosome dosage compensation"**
*Author: Lee J, et al. (2018)*
摘要：通过体外重组蛋白实验，证实MSL3L1与人类X染色体相关长链非编码RNA(如XIST)结合，推测其在哺乳动物剂量补偿机制中可能具有进化保守性功能。
3. **"Expression and purification of recombinant MSL3L1 for structural analysis"**
*Author: Gupta R, et al. (2019)*
摘要：开发了一种高效的真核表达系统(如HEK293细胞)纯化重组人MSL3L1蛋白，并通过结晶学初步解析其C端结构域，揭示了与MSL1蛋白结合的潜在位点。
4. **"Altered MSL3L1 expression in cancer: Implications for epigenetic therapies"**
*Author: Chen L, et al. (2021)*
摘要：在多种癌症中发现MSL3L1表达异常，体外实验显示其重组蛋白可调节肿瘤相关基因的乙酰化状态，提示其作为表观遗传治疗靶点的潜力。
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**注意**：以上文献为假设性示例，实际研究可能有限。建议通过PubMed、Google Scholar等平台以“MSL3L1”、“重组MSL3蛋白”或“MSL complex”为关键词检索最新文献。
MSL3L1 (Male-Specific Lethal 3-like 1) is a human homolog of the Drosophila MSL3 protein, a key component of the male-specific lethal (MSL) complex involved in dosage compensation. In fruit flies, the MSL complex ensures equal expression of X-linked genes between males (XY) and females (XX) by acetylating histone H4 at lysine 16 (H4K16ac), enhancing transcriptional output from the male X chromosome. While humans lack a direct dosage compensation mechanism, MSL3L1 retains functional links to chromatin modification and gene regulation. It associates with the human MSL complex, which includes MOF (histone acetyltransferase), to mediate H4K16 acetylation, influencing chromatin structure and transcriptional activation.
Recombinant MSL3L1 protein is artificially expressed in systems like E. coli or mammalian cells for functional studies. Its production enables exploration of its role in epigenetics, DNA repair, and cell differentiation. Research suggests MSL3L1 may regulate genes critical in development and cancer progression, with altered expression observed in malignancies. By studying recombinant MSL3L1. scientists aim to dissect its interactions within chromatin-modifying complexes and its potential as a therapeutic target. The protein’s structural analysis (e.g., chromodomain binding to methylated histones) further clarifies its role in epigenetic signaling. Ongoing research continues to unravel its contributions to cellular homeostasis and disease pathology.
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