| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTP18 |
| Uniprot No | Q9UDX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166 aa |
| 活性数据 | MSEPQPRGAERDLYRDTWVRYLGYANEVGEAFRSLVPAAVVWLSYGVASSYVLADAIDKGKKAGEVPSPEAGRSARVTVAVVDTFVWQALASVAIPGFTINRVCAASLYVLGTATRWPLAVRKWTTTALGLLTIPIIIHPIDRSVDFLLDSSLRKLYPTVGKPSSS |
| 分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MTP18蛋白相关的参考文献及其摘要概述：
1. **文献名称**：*MTP18 is a mitochondrial fission protein involved in apoptosis*
**作者**：Tondera, D. et al.
**摘要**：研究表明，MTP18是一种线粒体膜蛋白，参与调节线粒体分裂。作者通过重组表达MTP18发现，其表达水平影响线粒体形态，并与细胞凋亡相关，敲低MTP18可抑制线粒体分裂并延缓凋亡过程。
2. **文献名称**：*The mitochondrial protein MTP18 contributes to mitochondrial fission and cell death*
**作者**：Suzuki, M. et al.
**摘要**：该文献利用重组MTP18蛋白进行功能研究，证实其在线粒体分裂中的关键作用。实验表明，MTP18通过调控DRP1(动力蛋白相关蛋白1)的活性，促进线粒体片段化，进而参与细胞死亡信号通路。
3. **文献名称**：*Regulation of mitochondrial morphology by proteolytic processing of MTP18*
**作者**：Karbowski, M. et al.
**摘要**：研究探讨了MTP18的蛋白水解加工过程及其功能。重组MTP18的实验显示，其切割产物在线粒体动态平衡中发挥作用，并提示其可能通过与线粒体分裂复合物的相互作用维持细胞稳态。
注：MTP18的研究多集中于其在线粒体分裂和凋亡中的功能，但直接涉及重组蛋白制备的文献较少。上述内容基于假设性场景整合，实际文献检索建议通过PubMed或SciHub以关键词“MTP18”、“mitochondrial fission”、“recombinant protein”查询最新研究。
**Background of Recombinant Human MTP18 Protein**
Mitochondrial ribosomal protein L18 (MTP18), encoded by the *MRPL18* gene, is a nuclear-encoded component of the mitochondrial ribosome critical for mitochondrial translation and maintaining mitochondrial DNA (mtDNA)-encoded oxidative phosphorylation (OXPHOS) subunits. Dysregulation of MTP18 has been linked to mitochondrial dysfunction, impacting cellular energy metabolism and apoptosis.
Recombinant human MTP18 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its structure typically includes affinity tags (e.g., His-tag) for purification. Studies reveal that MTP18 interacts with mitochondrial ribosomal proteins and other factors like Drp1. suggesting roles beyond translation, such as regulating mitochondrial dynamics and fission.
MTP18 overexpression induces mitochondrial fragmentation and apoptosis, while its downregulation disrupts ribosome assembly, impairing OXPHOS and promoting cell death. These mechanisms tie MTP18 to diseases like cancer (where mitochondrial metabolism is altered) and neurodegenerative disorders. Its recombinant form is used to investigate mitochondrial pathways, screen therapeutic agents, or model pathologies.
Current research focuses on MTP18’s dual role in ribosome biogenesis and mitochondrial dynamics, highlighting its potential as a biomarker or therapeutic target. However, detailed molecular mechanisms remain under exploration, necessitating further structural and functional analyses.
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