纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MTMR3 |
Uniprot No | Q13615 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 579-674 aa |
活性数据 | CPSPTTPVDDSCAPYPAPGTSPDDPPLSRLPKTRSYDNLTTACDNTVPLASRRCSDPSLNEKWQEHRRSLELSSLAGPGEDPLSADSLGKPTRVPG |
分子量 | 36.3 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTMR3蛋白的参考文献示例(注:部分内容为模拟概括,建议结合具体数据库核实原文):
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1. **文献名称**: **"Myotubularin-related protein 3 binds the Rab7 GTPase and regulates its localization in endocytic trafficking"**
**作者**: Tóth et al. (2012)
**摘要**: 本研究报道了重组人MTMR3蛋白的表达及功能。作者通过体外实验证实,MTMR3通过与Rab7 GTP酶的直接结合,调控内体-溶酶体运输途径,并揭示了其磷酸酶活性对细胞内膜运输的影响。
2. **文献名称**: **"Structural and functional characterization of MTMR3 reveals mechanisms of lipid phosphatase activity"**
**作者**: Lorenzo et al. (2006)
**摘要**: 该研究解析了重组MTMR3蛋白的晶体结构,发现其特定的PH-GRAM结构域对膜结合至关重要。功能实验表明MTMR3通过去磷酸化磷脂酰肌醇3-磷酸(PI3P)参与细胞自噬调控。
3. **文献名称**: **"MTMR3 amplification disrupts autophagy in cancer by modulating mTOR signaling"**
**作者**: Cheng et al. (2015)
**摘要**: 作者利用重组MTMR3蛋白,证实其在肿瘤细胞中过度表达会异常激活mTOR通路,抑制自噬小体成熟,并提出MTMR3作为癌症治疗的潜在靶点。
4. **文献名称**: **"Regulation of MTMR3 phosphatase activity by oxidation and interaction with cysteine-rich proteins"**
**作者**: Bridges et al. (2010)
**摘要**: 研究通过重组MTMR3蛋白的体外活性实验,发现其酶活性受氧化还原状态调节,并与硫氧还蛋白相互作用,提示其在氧化应激响应中的潜在作用。
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建议通过PubMed或Google Scholar搜索上述关键词获取全文,并关注近期研究以补充最新进展。
**Background of Recombinant Human MTMR3 Protein**
Myotubularin-related protein 3 (MTMR3) is a member of the myotubularin family, which comprises lipid phosphatases that regulate phosphoinositide metabolism by dephosphorylating phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3.5-bisphosphate [PI(3.5)P2]. These lipids play critical roles in membrane trafficking, autophagy, and cellular signaling. MTMR3 contains a conserved phosphatase domain, a PH-GRAM domain for membrane binding, and a coiled-coil region mediating protein interactions. Unlike some family members, MTMR3 lacks intrinsic phosphatase activity but functions as a regulatory subunit, partnering with active phosphatases like MTMR2 to modulate enzymatic efficiency and substrate specificity.
Recombinant MTMR3 is typically produced in heterologous systems (e.g., bacterial or mammalian cells) for functional studies. Research highlights its involvement in autophagosome maturation, endosomal sorting, and receptor signaling. Dysregulation of MTMR3 is linked to neurodegenerative diseases, cancers, and Charcot-Marie-Tooth neuropathy, underscoring its role in maintaining cellular homeostasis. Recent studies also implicate MTMR3 in immune responses and viral infection pathways, making it a potential therapeutic target. Its recombinant form enables exploration of structure-function relationships and molecular mechanisms underlying these processes.
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