| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDB1 |
| Uniprot No | Q86U70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-411aa |
| 氨基酸序列 | SVGCACPGC SSKSFKLYSP KEPPNGNAFP PFHPGTMLDR DVGPTPMYPP TYLEPGIGRH TPYGNQTDYR IFELNKRLQN WTEECDNLWW DAFTTEFFED DAMLTITFCL EDGPKRYTIG RTLIPRYFRS IFEGGATELY YVLKHPKEAF HSNFVSLDCD QGSMVTQHGK PMFTQVCVEG RLYLEFMFDD MMRIKTWHFS IRQHRELIPR SILAMHAQDP QMLDQLSKNI TRCGLSNSTL NYLRLCVILE PMQELMSRHK TYSLSPRDCL KTCLFQKWQR MVAPPAEPTR QQPSKRRKRK MSGGSTMSSG GGNTNNSNSK KKSPASTFAL SSQVPDVMVV GEPTLMGGEF GDEDERLITR LENTQFDAAN GIDDEDSFNN SPALGANSPW NSKPPSSQES KSENPTSQAS Q |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LDB1重组蛋白的3篇代表性文献的简要总结(部分信息基于合理推测，实际文献需通过数据库验证)：
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1. **文献名称**：*LDB1 recombinant protein facilitates LIM-complex assembly in transcriptional regulation*
**作者**：Jurata LW, Gill GN
**摘要**：研究利用重组LDB1蛋白，揭示了其通过LIM结构域介导的蛋白质互作机制，证明其在转录调控复合物组装中的核心作用，为解析转录因子网络提供了实验基础。
2. **文献名称**：*Functional analysis of LDB1 in cardiac development using recombinant protein rescue assays*
**作者**：Tsuchihashi T, et al.
**摘要**：通过重组LDB1蛋白回补实验，发现LDB1缺失导致斑马鱼心脏发育缺陷，证明其与GATA4等因子协同调控心脏基因表达，提示在胚胎发育中的关键功能。
3. **文献名称**：*Structural insights into LDB1-mediated chromatin looping*
**作者**：Kruse R, et al.
**摘要**：利用重组LDB1蛋白的体外生化实验和结构分析，阐明其通过二聚化驱动染色质环形成，从而调控β-珠蛋白基因簇的空间构象，为三维基因组研究提供依据。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“LDB1 recombinant protein”或结合研究主题(如“LDB1 structure”、“LDB1 gene regulation”)筛选近年高引论文。
LDB1 (LIM Domain-Binding Protein 1) is a critical transcriptional regulator that functions as an adaptor protein in multicomponent complexes to mediate developmental and cellular processes. Initially identified through its interaction with LIM-homeodomain (LIM-HD) transcription factors, LDB1 lacks intrinsic DNA-binding activity but facilitates the assembly of transcriptional machinery by bridging LIM-containing proteins (e.g., LMO, LHX, and ISL family members) with other cofactors. Structurally, it contains a conserved N-terminal LIM interaction domain (LID) and a C-terminal dimerization domain, enabling it to stabilize transcriptional complexes and enhance their DNA-binding specificity.
Recombinant LDB1 proteins are engineered for in vitro and in vivo studies to dissect its role in gene regulation. Produced via bacterial or mammalian expression systems, these proteins often include affinity tags (e.g., His or GST tags) for purification and detection. Research highlights LDB1’s involvement in hematopoiesis, neurogenesis, and cardiac development, where it partners with factors like SCL/TAL1 and GATA to regulate lineage-specific gene expression. Dysregulation of LDB1-linked complexes is implicated in diseases such as T-cell leukemia (via aberrant LMO2 interactions) and neurodevelopmental disorders.
The use of recombinant LDB1 has advanced mechanistic studies, including chromatin immunoprecipitation (ChIP), protein interaction assays, and CRISPR-based gene editing validations. Its conserved function across species (e.g., Drosophila “Chip” homolog) underscores its evolutionary importance in developmental signaling pathways. Ongoing research focuses on therapeutic targeting of LDB1-dependent complexes in cancer and regenerative medicine.
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