Recombinant Human LYAR protein

**Background of LYAR Recombinant Protein**

LYAR (Ly1 Antibiotic Resistance homolog), also known as cell proliferation-regulating nucleolar protein, is a zinc finger-containing protein encoded by the *LYAR* gene in humans. Initially identified in mice as a gene associated with cell growth and differentiation, LYAR is evolutionarily conserved and plays multifaceted roles in cellular processes, including ribosome biogenesis, cell cycle regulation, and apoptosis. Structurally, LYAR contains a C2H2-type zinc finger motif critical for DNA/RNA binding and a nucleolar localization signal, reflecting its involvement in ribosome assembly and nucleolar functions.

Studies highlight LYAR's dual role in both promoting cell proliferation and suppressing apoptosis, particularly in stem cells and cancer. It regulates the stability of specific mRNAs and interacts with ribosomal proteins, influencing translation efficiency. In embryonic development, LYAR is essential for proper tissue formation, as knockout models exhibit developmental defects.

In cancer biology, LYAR is frequently overexpressed in malignancies such as leukemia, hepatocellular carcinoma, and glioblastoma, correlating with poor prognosis. Its oncogenic potential is linked to enhancing cancer cell survival, metastasis, and chemoresistance. Consequently, LYAR is investigated as a therapeutic target, with inhibitors under exploration.

Recombinant LYAR protein, produced via bacterial or mammalian expression systems, enables functional studies. It retains DNA/RNA-binding activity and serves as a tool to elucidate molecular mechanisms, screen inhibitors, or develop diagnostic assays. Researchers also utilize LYAR recombinant proteins to study its interaction with ribosomal components or apoptotic regulators, providing insights into ribosomopathies and cancer progression.

Overall, LYAR's diverse roles in cell homeostasis and disease underscore its significance in basic and translational research, driving interest in recombinant protein applications for mechanistic and therapeutic discoveries.