Recombinant Human LYAR protein

中文名： Ly1抗体反应同源物(LYAR)重组蛋白
别名： LYAR；Cell growth-regulating nucleolar protein

货号: PA1000-7819

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	LYAR
Uniprot No	Q9NX58
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-379aa
氨基酸序列	MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGGKGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKNSLKVHNESILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEISTKVPASKVKDAVEQQGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPEANGSAGKRSKKKKQRKDSASEEEAHVGAGKRKRRHSEVETDSKKKKMKLPEHPEGGEPEDDEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRSEEELLVIFNKKISKNPTFKLLKDKVKLVK
预测分子量	45.6kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于LYAR重组蛋白的3篇参考文献的简要列举：

1. **标题**：Ly-1 antibody reactive clone is an important nucleolar protein in control of cell proliferation

**作者**：Wang Y, et al.

**摘要**：研究揭示了LYAR重组蛋白作为核仁调控因子的功能，通过调控核糖体RNA合成影响细胞增殖，并证明其在小鼠胚胎干细胞分化中起关键作用。

2. **标题**：LYAR interacts with the NuRD complex to regulate transcription of hematopoietic factors

**作者**：Li S, et al.

**摘要**：该文献报道LYAR重组蛋白通过结合NuRD染色质重塑复合物，调控造血相关基因的转录，实验表明LYAR缺失导致小鼠造血干细胞分化异常。

3. **标题**：Oncogenic role of LYAR in human colorectal cancer via promoting EMT

**作者**：Zhang R, et al.

**摘要**：研究发现LYAR重组蛋白在结直肠癌中高表达，通过激活上皮间质转化(EMT)通路促进肿瘤侵袭转移，体外实验证实其重组蛋白可增强癌细胞迁移能力。

注：以上文献信息为模拟示例，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science检索关键词“LYAR recombinant protein”获取最新研究。

背景信息

**Background of LYAR Recombinant Protein**

LYAR (Ly1 Antibiotic Resistance homolog), also known as cell proliferation-regulating nucleolar protein, is a zinc finger-containing protein encoded by the *LYAR* gene in humans. Initially identified in mice as a gene associated with cell growth and differentiation, LYAR is evolutionarily conserved and plays multifaceted roles in cellular processes, including ribosome biogenesis, cell cycle regulation, and apoptosis. Structurally, LYAR contains a C2H2-type zinc finger motif critical for DNA/RNA binding and a nucleolar localization signal, reflecting its involvement in ribosome assembly and nucleolar functions.

Studies highlight LYAR's dual role in both promoting cell proliferation and suppressing apoptosis, particularly in stem cells and cancer. It regulates the stability of specific mRNAs and interacts with ribosomal proteins, influencing translation efficiency. In embryonic development, LYAR is essential for proper tissue formation, as knockout models exhibit developmental defects.

In cancer biology, LYAR is frequently overexpressed in malignancies such as leukemia, hepatocellular carcinoma, and glioblastoma, correlating with poor prognosis. Its oncogenic potential is linked to enhancing cancer cell survival, metastasis, and chemoresistance. Consequently, LYAR is investigated as a therapeutic target, with inhibitors under exploration.

Recombinant LYAR protein, produced via bacterial or mammalian expression systems, enables functional studies. It retains DNA/RNA-binding activity and serves as a tool to elucidate molecular mechanisms, screen inhibitors, or develop diagnostic assays. Researchers also utilize LYAR recombinant proteins to study its interaction with ribosomal components or apoptotic regulators, providing insights into ribosomopathies and cancer progression.

Overall, LYAR's diverse roles in cell homeostasis and disease underscore its significance in basic and translational research, driving interest in recombinant protein applications for mechanistic and therapeutic discoveries.