| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATR3 |
| Uniprot No | P43243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-847aa |
| 氨基酸序列 | MSKSFQQSSLSRDSQGHGRDLSAAGIGLLAAATQSLSMPASLGRMNQGTARLASLMNLGMSSSLNQQGAHSALSSASTSSHNLQSIFNIGSRGPLPLSSQHRGDADQASNILASFGLSARDLDELSRYPEDKITPENLPQILLQLKRRRTEEGPTLSYGRDGRSATREPPYRVPRDDWEEKRHFRRDSFDDRGPSLNPVLDYDHGSRSQESGYYDRMDYEDDRLRDGERCRDDSFFGETSHNYHKFDSEYERMGRGPGPLQERSLFEKKRGAPPSSNIEDFHGLLPKGYPHLCSICDLPVHSNKEWSQHINGASHSRRCQLLLEIYPEWNPDNDTGHTMGDPFMLQQSTNPAPGILGPPPPSFHLGGPAVGPRGNLGAGNGNLQGPRHMQKGRVETSRVVHIMDFQRGKNLRYQLLQLVEPFGVISNHLILNKINEAFIEMATTEDAQAAVDYYTTTPALVFGKPVRVHLSQKYKRIKKPEGKPDQKFDQKQELGRVIHLSNLPHSGYSDSAVLKLAEPYGKIKNYILMRMKSQAFIEMETREDAMAMVDHCLKKALWFQGRCVKVDLSEKYKKLVLRIPNRGIDLLKKDKSRKRSYSPDGKESPSDKKSKTDGSQKTESSTEGKEQEEKSGEDGEKDTKDDQTEQEPNMLLESEDELLVDEEEAAALLESGSSVGDETDLANLGDVASDGKKEPSDKAVKKDGSASAAAKKKLKKVDKIEELDQENEAALENGIKNEENTEPGAESSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MATR3重组蛋白的3篇代表性文献的简要总结(文献标题和作者为虚构示例，仅供格式参考)：
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1. **文献名称**: "MATR3 regulates RNA splicing through its interaction with nuclear matrix components"
**作者**: Zhang Y, et al.
**摘要**: 本研究利用重组MATR3蛋白揭示了其通过结合核基质中的RNA和蛋白质复合物调控pre-mRNA剪接的分子机制，证明MATR3的锌指结构域对维持剪接体稳定性至关重要。
2. **文献名称**: "Cryo-EM structure of the MATR3 amyloidogenic region reveals pathogenic aggregation propensity"
**作者**: Lee S, et al.
**摘要**: 通过重组表达人源MATR3蛋白的C端结构域并解析其冷冻电镜结构，发现该区域在神经退行性疾病(如ALS)中易形成病理性淀粉样纤维聚集，为疾病机制研究提供结构基础。
3. **文献名称**: "MATR3 interacts with TDP-43 and modulates its cytoplasmic mislocalization in neurodegeneration"
**作者**: Maruyama H, et al.
**摘要**: 重组蛋白互作实验表明，MATR3与TDP-43在细胞核内形成复合物，抑制后者在应激条件下的胞质错误定位，提示MATR3功能缺失可能导致运动神经元变性疾病的病理过程。
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注：以上内容为简化示例，实际文献需通过PubMed或Google Scholar等数据库检索关键词“MATR3 recombinant protein”获取。真实研究多聚焦于MATR3在RNA代谢、神经退行性疾病及癌症中的功能。
MATR3 (Matrin 3) is a multifunctional nuclear matrix protein encoded by the *MATR3* gene in humans. It is primarily localized in the nuclear matrix and plays critical roles in DNA repair, RNA metabolism, chromatin organization, and transcriptional regulation. Structurally, MATR3 contains two zinc finger domains and two RNA recognition motifs (RRMs), enabling interactions with both DNA and RNA. This protein is highly expressed in neuronal tissues and has been implicated in neurodegenerative diseases, particularly amyotrophic lateral sclerosis (ALS) and distal myopathy, where mutations in *MATR3* are linked to disease pathogenesis.
The interest in recombinant MATR3 protein stems from its association with cellular stress responses and disease mechanisms. Studies show that mutant MATR3 forms abnormal aggregates in neuronal cells, disrupting RNA processing and nucleocytoplasmic transport, which are hallmarks of ALS. Recombinant MATR3 is commonly produced in *E. coli* or mammalian expression systems for in vitro studies to elucidate its molecular interactions, post-translational modifications, and pathogenic mutations. Researchers use it to investigate binding partners, such as TDP-43 or FUS, which are also linked to neurodegeneration.
Additionally, recombinant MATR3 serves as a tool for structural studies, including crystallography and cryo-EM, to map functional domains and mutation sites. Its therapeutic potential is being explored, with efforts to develop small molecules or gene therapies targeting MATR3 dysfunction. Overall, MATR3 recombinant protein is a vital resource for understanding its biological roles and developing strategies to combat related diseases.
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