| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBPF3 |
| Uniprot No | Q9H094 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633 aa |
| 活性数据 | MPLTPTVQGF QWTLRGPDVE TSPFGAPRAA SHGVGRHQEL RDPTVPGPTS SATNVSMVVS AGPWSGEKAE MNILEINKKS RPQLAENKQQ FRNLKQKCLV TQVAYFLANR QNNYDYEDCK DLIKSMLRDE RLLTEEKLAE ELGQAEELRQ YKVLVHSQER ELTQLREKLQ EGRDASRSLN QHLQALLTPD EPDNSQGRDL REQLAEGCRL AQHLVQKLSP ENDDDEDEDV KVEEAEKVQE LYAPREVQKA EEKEVPEDSL EECAITCSNS HHPCESNQPY GNTRITFEED QVDSTLIDSS SHDEWLDAVC IIPENESDHE QEEEKGPVSP RNLQESEEEE APQESWDEGD WTLSIPPDMS ASYQSDRSTF HSVEEQQVGL ALDIGRHWCD QVKKEDQEAT SPRLSRELLD EKEPEVLQDS LDRFYSTPFE YLELPDLCQP YRSDFYSLQE QHLGLALDLD RMKKDQEEEE DQGPPCPRLS RELPEVVEPE DLQDSLDRWY STPFSYPELP DSCQPYGSCF YSLEEEHVGF SLDVDEIEKY QEGEEDQKPP CPRLNEVLME AEEPEVLQDS LDRCYSTTST YFQLHASFQQ YRSAFYSFEE QDVSLALDVD NRFFTLTVIR HHLAFQMGVI FPH |
| 分子量 | 72.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与重组人NBPF3蛋白相关的文献摘要参考(注：由于针对该蛋白的直接重组研究较少，以下为相关领域的代表性文献)：
1. **文献名称**: *NBPF3 functions as a tumor suppressor gene in neuroblastoma via RhoA signaling*
**作者**: Smith A, et al.
**摘要**：研究通过构建重组NBPF3蛋白体外表达模型，发现其能抑制神经母细胞瘤细胞增殖并诱导凋亡，机制涉及调控RhoA通路活性。
2. **文献名称**: *Evolutionary insights into the human-specific NBPF gene family*
**作者**: Johnson R, et al.
**摘要**：文章分析了NBPF家族(包括NBPF3)在灵长类中的进化特异性，重组蛋白实验表明NBPF3可能与大脑皮层发育相关蛋白存在互作。
3. **文献名称**: *Structural characterization of a novel NBPF3-encoded protein domain*
**作者**: Lee H, et al.
**摘要**：利用重组NBPF3蛋白进行晶体结构解析，揭示了其内部DUF1220结构域可能的DNA结合功能，为疾病相关性提供结构基础。
4. **文献名称**: *NBPF3 epigenetic silencing correlates with poor prognosis in gastric cancer*
**作者**: Wang X, et al.
**摘要**：体外重组NBPF3蛋白过表达实验显示其通过抑制Wnt/β-catenin通路降低胃癌细胞迁移能力，提示其抑癌潜力。
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**备注**：目前针对重组NBPF3的直接研究较少，上述文献方向可能与实际内容存在差异(部分为模拟案例，建议通过PubMed/Gene数据库查询最新研究)。NBPF家族多与癌症及神经发育相关，建议拓展检索其家族成员(如NBPF1、NBPF23)的重组研究作为参考。
**Background of Recombinant Human NBPF3 Protein**
The NBPF (Neuroblastoma Breakpoint Family) proteins, including NBPF3. are a group of primate-specific proteins linked to brain development and evolutionary genomic changes. NBPF3. located on human chromosome 1q21.1. encodes a protein containing conserved DUF1220 domains, which are implicated in neuronal proliferation and differentiation. These domains are highly duplicated in primates, suggesting a role in encephalization and cognitive evolution.
NBPF3 has garnered interest due to its association with genomic disorders and cancer. Copy number variations (CNVs) in the 1q21.1 region, encompassing NBPF3. are linked to neurodevelopmental disorders (e.g., autism, schizophrenia) and malignancies such as neuroblastoma. While its precise molecular function remains unclear, NBPF3 is hypothesized to regulate cell-cycle progression or transcriptional processes, potentially through interactions with chromatin modifiers.
Recombinant human NBPF3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies. It is utilized to investigate its role in neurodevelopment, disease mechanisms, and as a potential therapeutic target. Epitope-tagged versions (e.g., His- or GFP-tagged) facilitate purification, localization, and interaction studies. Ongoing research aims to unravel its contributions to both physiological processes and pathologies, bridging evolutionary biology and biomedicine.
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