| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBPF15 |
| Uniprot No | Q8N660 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670 aa |
| 活性数据 | MVVSAGPLSS EKAEMNILEI NEKLRPQLAE KKQQFRNLKE KCFLTQLAGF LANRQKKYKY EECKDLIKFM LRNERQFKEE KLAEQLKQAE ELRQYKVLVH AQERELTQLR EKLREGRDAS RSLNEHLQAL LTPDEPDKSQ GQDLQEQLAE GCRLTQHLVQ KLSPENDNDD DEDVQVEVAE KVQKSSAPRE MQKAEEKEVP EDSLEECAIT CSNSHGPYDS NQPHKKTKIT FEEDKVDSTL IGSSSHVEWE DAVHIIPENE SDDEEEEEKG PVSPRNLQES EEEEVPQESW DEGYSTLSIP PEMLASYQSY SSTFHSLEEQ QVCMAVDIGR HRWDQVKKED QEATGPRLSR ELLDEKEPEV LQDSLDRCYS TPSGCLELTD SCQPYRSAFY VLEQQRVGLA IDMDEIEKYQ EVEEDQDPSC PRLSRELLDE KEPEVLQDSL DRCYSTPSDY LELPDLGQPY SSAVYSLEEQ YLGLALDVDR IKKDQEEEED QGPPCPRLSR ELLEVVEPEV LQDSLDRCYS TPSSCLEQPD SCQPYGSSFY ALEEKHVGFS LDVGEIEKKG KGKKRRGRRS KKKRRRGRKE GEDDNPPCPR LYGVLMEVEE PEVLQDSLDR CYSTPSMYFE QPDSFQHYRS VFYSFEEEHI SFALYVDNRF FTLTVTSLHL VFQMGVIFPQ |
| 分子量 | 77.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NBPF15蛋白的3篇假设参考文献示例(注：实际文献可能有限，以下为基于NBPF家族相关研究构建的示例)：
1. **文献名称**：Expression and Purification of Recombinant Human NBPF15 Protein in E. coli
**作者**：Zhang Y. et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人NBPF15蛋白，通过His标签亲和层析和尺寸排阻色谱优化纯化条件，验证了蛋白的稳定性和生物活性。
2. **文献名称**：NBPF15 Associates with Neurodevelopmental Disorders: Functional Analysis in Cortical Neurons
**作者**：Chen L. & Patel R.
**摘要**：研究发现重组NBPF15蛋白在体外神经元分化中调控突触形成相关基因表达，提示其在神经发育障碍(如自闭症)中的潜在病理机制。
3. **文献名称**：Structural Insights into NBPF15 Domains and Their Evolution in Primates
**作者**：Smith J. et al.
**摘要**：通过X射线晶体学解析了NBPF15蛋白的部分结构域，揭示其与灵长类特异性进化相关，可能参与人类特有的认知功能调控。
**提示**：
NBPF15实际研究可能较少，上述文献为模拟示例。建议通过PubMed或Google Scholar检索最新文献，或扩大关键词至“NBPF family”或结合疾病关联(如“cancer”“neurodevelopment”)进行扩展搜索。
**Background of Recombinant Human NBPF15 Protein**
The NBPF (Neuroblastoma Breakpoint Family) proteins, encoded by the NBPF gene family, are implicated in neurodevelopment and cancer biology. Human NBPF15. a member of this family, is associated with chromosomal breakpoints observed in neuroblastoma, a pediatric neural crest tumor. The NBPF family is characterized by conserved DUF1220 protein domains, which are linked to brain evolution and cortical neuron proliferation. NBPF15. located on chromosome 1. exhibits copy number variations (CNVs) correlated with neurological disorders and cancer susceptibility.
Recombinant human NBPF15 protein is engineered *in vitro* using bacterial or mammalian expression systems, enabling functional studies. This purified protein aids in investigating its role in cellular processes, such as differentiation, apoptosis, and genomic stability. Research suggests NBPF15 may act as a tumor suppressor or modulator of transcriptional regulation, though its precise mechanisms remain under exploration. Its recombinant form is utilized in antibody development, protein interaction assays, and disease modeling, particularly in neuroblastoma and neurodevelopmental conditions.
Current studies focus on elucidating NBPF15's involvement in neural plasticity, cancer pathways, and potential therapeutic applications. Its unique domain architecture and disease associations make it a compelling target for biomedical research.
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