| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBPF1 |
| Uniprot No | Q3BBV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-603 aa |
| 活性数据 | MLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASCSLNQHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSAPREMPKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYKSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVDNRFLTLMGRSLHLVFQMGVIFPQ |
| 分子量 | 66.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NBPF1蛋白的参考文献及其简要摘要：
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1. **标题**：*Recombinant NBPF1 Protein Enhances Neuronal Differentiation via Wnt Signaling*
**作者**：Smith A, et al. (2021)
**摘要**：研究发现重组NBPF1蛋白在神经母细胞瘤细胞中通过激活Wnt/β-catenin通路，显著促进神经元分化和抑制肿瘤生长。
2. **标题**：*Structural Insights into Human NBPF1 and Primate-Specific Evolution*
**作者**：Zhang L, et al. (2019)
**摘要**：解析了重组NBPF1蛋白的晶体结构，发现其DUF1220结构域在灵长类进化中高度扩增，可能参与大脑发育调控。
3. **标题**：*Recombinant NBPF1 as a Therapeutic Candidate in Neuroblastoma*
**作者**：Johnson R, et al. (2020)
**摘要**：开发了重组NBPF1的高效表达系统，并在体外实验中证实其诱导神经母细胞瘤细胞凋亡的能力，提示潜在治疗价值。
4. **标题**：*NBPF1 Modulates Chromatin Remodeling in Cortical Neurons*
**作者**：Chen M, et al. (2022)
**摘要**：重组NBPF1蛋白通过与BRD4等表观遗传因子相互作用，调控皮层神经元发育相关基因的染色质开放性和转录活性。
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**注意**：上述文献为模拟示例，实际研究需通过学术数据库检索验证。建议使用PubMed或Google Scholar以关键词“recombinant NBPF1”或“NBPF1 function”查找最新文献。
**Background of Recombinant Human NBPF1 Protein**
The Neuroblastoma Breakpoint Family (NBPF) member 1 (NBPF1) is a human-specific protein encoded by the *NBPF1* gene, located on chromosome 1. This gene belongs to the NBPF family, characterized by evolutionarily conserved DUF1220 protein domains linked to brain development and cortical expansion. NBPF1 has gained attention due to its potential role in neurodevelopment, with studies suggesting involvement in neural cell proliferation, differentiation, and synaptic plasticity.
Recombinant NBPF1 protein is artificially synthesized using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian cell systems. Its production enables functional studies to unravel mechanisms in neurodevelopmental disorders, cancers, and cognitive evolution. Notably, genomic alterations in *NBPF1* (e.g., deletions or duplications) are implicated in neuroblastoma, autism spectrum disorders, and schizophrenia, highlighting its clinical relevance.
Despite its association with critical biological processes, NBPF1's precise molecular functions remain partially elusive. Structural analyses reveal tandem repeats of DUF1220 domains, suggesting roles in protein-protein interactions or signaling pathways. Current research focuses on its influence on neuronal networks, potential therapeutic applications, and evolutionary significance in primate brain complexity. Recombinant NBPF1 serves as a vital tool for *in vitro* assays, antibody development, and drug screening, bridging gaps between genetic insights and functional understanding.
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