| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBEA |
| Uniprot No | Q8NFP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1133-1220 aa |
| 活性数据 | ADEKEDLPNSSTSFLFDKIPKQEEKLLPELSSNHIIPNIQDTQVHLGVSDDLGLLAHMTGSVDLTCTSSIIEEKEFKIHTTSDGMSSI |
| 分子量 | 35.42 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NBEA(Neurobeachin)蛋白的3篇示例参考文献及摘要概括：
1. **文献名称**：*"Expression and Purification of Recombinant Human Neurobeachin in E. coli for Functional Studies"*
**作者**：Wang, Y. et al.
**摘要**：该研究报道了在大肠杆菌中高效表达并纯化重组人NBEA蛋白的方法，通过优化诱导条件和层析技术获得高纯度蛋白，并证实其与突触蛋白的体外结合活性，为后续功能研究奠定基础。
2. **文献名称**：*"Neurobeachin Regulates Synaptic Vesicle Trafficking via Rab11 Interaction"*
**作者**：Smith, J.R. & Müller, U.
**摘要**：利用重组人NBEA蛋白进行体外互作实验，发现其通过BEACH结构域与Rab11 GTP酶结合，调控突触囊泡的运输和神经递质释放，揭示了NBEA在突触功能中的关键作用。
3. **文献名称**：*"Autism-Associated NBEA Mutants Disrupt Postsynaptic Protein Complexes"*
**作者**：Zhang, L. et al.
**摘要**：研究通过表达携带自闭症相关突变的重组NBEA蛋白，发现其无法与PSD-95等突触后支架蛋白结合，导致神经元形态异常，表明NBEA突变可能通过破坏突触后复合物参与疾病机制。
4. **文献名称**：*"Structural Insights into the Neurobeachin PH Domain and Its Role in Membrane Association"*
**作者**：Li, H. et al.
**摘要**：通过解析重组人NBEA蛋白PH结构域的晶体结构，发现其与磷脂酰肌醇的结合模式，揭示了NBEA靶向细胞膜并参与膜重塑的分子基础，为理解其神经功能提供结构依据。
---
示例文献为虚构，实际研究需查询PubMed或Google Scholar等数据库。
Neurobeachin (NBEA) is a large multidomain protein encoded by the *NBEA* gene, primarily expressed in neuronal tissues and endocrine cells. It belongs to the BEACH domain-containing protein family, known for roles in vesicle trafficking, membrane dynamics, and synaptic regulation. Structurally, NBEA contains conserved domains like a PH domain, a BEACH domain, and WD40 repeats, which mediate lipid binding, protein interactions, and scaffolding functions. Research links NBEA to neuronal development, particularly in neurotransmitter release, synaptic plasticity, and the formation of excitatory synapses.
Mutations or deletions in *NBEA* are associated with neurodevelopmental disorders, including autism spectrum disorders (ASD) and intellectual disabilities. Studies in *NBEA*-knockout mice reveal disrupted synaptic architecture, impaired secretion in endocrine cells, and behavioral anomalies. Recombinant human NBEA protein, typically produced in mammalian or insect expression systems, retains post-translational modifications critical for its function. It serves as a vital tool for *in vitro* studies, such as investigating synaptic protein interactions, vesicle transport mechanisms, or screening therapeutic agents targeting NBEA-related pathways. Its applications extend to structural biology (e.g., crystallography) and antibody development for diagnostic research. Current challenges include optimizing recombinant NBEA stability and activity for functional assays, given its large size (~300 kDa) and complex domain organization.
×
