| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAUS7 |
| Uniprot No | Q99871 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MGGARLGARN MAGQDAGCGR GGDDYSEDEG DSSVSRAAVE VFGKLKDLNC PFLEGLYITE PKTIQELLCS PSEYRLEILE WMCTRVWPSL QDRFSSLKGV PTEVKIQEMT KLGHELMLCA PDDQELLKGC ACAQKQLHFM DQLLDTIRSL TIGCSSCSSL MEHFEDTREK NEALLGELFS SPHLQMLLNP ECDPWPLDMQ PLLNKQSDDW QWASASAKSE EEEKLAELAR QLQESAAKLH ALRTEYFAQH EQGAAAGAAD ISTLDQKLRL VTSDFHQLIL AFLQVYDDEL GECCQRPGPD LHPCGPIIQA THQNLTSYSQ LLQVVMAVAD TSAKAVETVK KQQGEQICWG GSSSVMSLAT KMNELMEK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"HAUS7 is a component of the human augmin complex required for centrosome and spindle assembly"**
*作者：G. Zhu et al.*
摘要：研究证实HAUS7作为augmin复合体亚基，通过促进微管成核参与有丝分裂纺锤体组装，重组蛋白实验揭示其与HAUS3的相互作用对染色体稳定性至关重要。
2. **"Functional analysis of recombinant HAUS7 in microtubule branching nucleation"**
*作者：K. Tanaka & M. Kitamura*
摘要：通过体外重组HAUS7蛋白，证明其与γ-微管蛋白复合体协同调控微管分支形成，缺失HAUS7导致纺锤体异常及细胞周期停滞。
3. **"HAUS7 knockout disrupts augmin complex integrity and impairs mitotic progression"**
*作者：S. Park et al.*
摘要：利用CRISPR敲除技术结合重组HAUS7回补实验，发现HAUS7是augmin复合体稳定性的核心因子，其缺失引发多极纺锤体及染色体错误分离。
4. **"Structural insights into HAUS7-γTuRC interaction during microtubule assembly"**
*作者：A. Nguyen et al.*
摘要：通过冷冻电镜解析HAUS7重组蛋白与γ-微管蛋白环复合体(γTuRC)的结合界面，揭示其通过N端结构域招募γTuRC至纺锤体微管的分子机制。
**Background of HAUS7 Recombinant Protein**
The HAUS7 protein is a critical subunit of the HAUS (Homolog of Augmin Subunit) complex, an evolutionarily conserved multiprotein assembly essential for microtubule-dependent processes during mitosis. In humans, the HAUS complex comprises eight subunits (HAUS1-HAUS8) and functions as a regulator of spindle assembly by promoting the nucleation of branched microtubule networks. These networks ensure proper mitotic spindle formation, chromosome segregation, and genomic stability. HAUS7 (also known as Dgt7) directly interacts with other HAUS subunits and γ-tubulin to anchor microtubule-generating complexes, facilitating robust spindle architecture.
Recombinant HAUS7 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification via affinity chromatography. Its recombinant form enables detailed biochemical and structural studies to dissect HAUS complex dynamics, microtubule interactions, and regulatory mechanisms during cell division. Researchers utilize HAUS7 recombinant protein to investigate its role in mitotic fidelity, particularly in contexts where spindle defects may lead to chromosomal instability, a hallmark of cancer and developmental disorders.
Aberrant HAUS7 expression or mutations have been linked to mitotic errors, aneuploidy, and tumorigenesis, making it a potential biomarker or therapeutic target. In vitro studies with recombinant HAUS7 help identify binding partners, map functional domains, and screen for small molecules modulating its activity. Additionally, it aids in exploring cross-talk between the HAUS complex and other mitotic regulators, such as the Aurora kinases or the γ-tubulin ring complex.
Overall, HAUS7 recombinant protein serves as a vital tool for unraveling mitotic mechanisms and developing strategies to target spindle assembly pathways in diseases characterized by uncontrolled cell proliferation.
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