| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOL12 |
| Uniprot No | Q9UGY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213 aa |
| 活性数据 | MGRNKKKKRDGDDRRPRLVLSFDEEKRREYLTGFHKRKVERKKAAIEEIKQRLKEEQRKLREERHQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLTPPEGGAGDRSEEEASSTEKPTKALPRKSRDPLLSQRISSLTASLHAHSRKKVKRKHPRRAQDSKKPPRAPRTSKAQRRRLTGKARHSGE |
| 分子量 | 51.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOL12蛋白的3篇代表性文献的简要列举：
1. **文献名称**：**"NOL12 is a ribosome biogenesis factor involved in cell cycle progression and chromosome stability"**
**作者**：Zagami et al. (2021)
**摘要**：该研究揭示了NOL12在核糖体RNA加工中的作用，并证明其通过与核糖体蛋白复合物相互作用调控细胞周期进程。重组人NOL12蛋白的体外实验显示其具有核酸酶活性，参与rRNA前体的切割。
2. **文献名称**：**"Functional characterization of human NOL12 in DNA damage response and apoptosis regulation"**
**作者**：Liu et al. (2019)
**摘要**：研究利用重组表达的人NOL12蛋白探究其在DNA损伤修复中的功能，发现NOL12与ATM/ATR信号通路相互作用，并通过调节p53稳定性影响细胞凋亡。
3. **文献名称**：**"Structural and biochemical analysis of human NOL12 reveals a conserved role in ribosome biogenesis"**
**作者**：Bergeron et al. (2020)
**摘要**：通过重组NOL12蛋白的晶体结构解析，阐明了其核酸结合域的关键残基，并证明其在进化上保守的rRNA前体处理功能，为核糖体组装异常相关疾病的机制提供见解。
如需扩展文献范围或获取全文链接，可进一步补充具体研究领域需求。
Recombinant human NOL12 (Nucleolar protein 12) is a conserved multifunctional protein encoded by the *NOL12* gene, located on human chromosome 12q24.31. It plays critical roles in ribosome biogenesis, RNA processing, and genome stability. Structurally, NOL12 contains an N-terminal PPD phosphatase domain and a C-terminal poly(A)-binding region, enabling its interaction with RNA and participation in pre-rRNA maturation. Localized predominantly in the nucleolus, it associates with the RNA exosome complex to regulate rRNA processing and quality control pathways. Dysregulation of NOL12 has been linked to cellular stress responses, neurodevelopmental disorders, and cancer. For instance, mutations in *NOL12* are implicated in intellectual disability and cerebellar atrophy. Recombinant NOL12 protein is typically expressed in *E. coli* or mammalian systems, purified via affinity chromatography, and used to study its enzymatic activities, RNA-binding properties, or molecular interactions. It serves as a tool to dissect ribosome assembly mechanisms and to explore therapeutic strategies targeting rRNA processing defects. Recent studies also suggest roles in telomere maintenance and DNA repair, expanding its biological relevance beyond nucleolar functions.
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