| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOBOX |
| Uniprot No | O60393 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-691 aa |
| 活性数据 | MALLLTLTSP DLEGTWDTRD KDGFKAQEGP PLAVPEFPVC GLYRIYGVCG SFSSFFIIRC SLCALETLKS PQHDPLEIPE QSLKLIPLVS GKRELTRGQK AGEKPLAAGP GEEELLRGSA PHAQDTQSEE LPPSCTISGE KKPPAVSGEA TGADAGRLCP PPRSRAPHKD RTLARSRPQT QGEDCSLPVG EVKIGKRSYS PAPGKQKKPN AMGLAPTSSP GAPNSARATH NPVPCGSGRG PCHLANLLST LAQSNQNRDH KQGPPEVTCQ IRKKTRTLYR SDQLEELEKI FQEDHYPDSD KRREIAQTVG VTPQRIMVKG AGSLVAGWSG GGPTIETLEL QSERSAVAWV WFQNRRAKWR KMEKLNGKES KDNPAAPGPA SSQCSSAAEI LPAVPMEPKP DPFPQESPLD TFPEPPMLLT SDQTLAPTQP SEGAQRVVTP PLFSPPPVRR ADLPFPLGPV HTPQLMPLLM DVAGSDSSHK DGPCGSWGTS ITLPPPCSYL EELEPQDYQQ SNQPGPFQFS QAPQPPLFQS PQPKLPYLPT FPFSMPSSLT LPPPEDSLFM FPCGPSGGTS QGYCPGASSG QILMQPPAGN IGTASWSDPC LPELPFPGPF CPQALGHPPG GDGYFPDLFP TPCPQALGRQ PSSALSWMPE GARPGTGPLL SKAKEEPPAA SLDQPSALEE ARGDDKNSHV P |
| 分子量 | 73.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOBOX蛋白的虚构参考文献示例(注：文献为模拟生成，非真实存在)：
1. **文献名称**：Functional Characterization of Recombinant Human NOBOX Protein in Oocyte Maturation
**作者**：Liu Y, et al.
**摘要**：本研究通过原核系统成功表达并纯化了重组人NOBOX蛋白，发现其通过结合卵母细胞特异性启动子调控关键基因(如Gdf9和Bmp15)的转录，并证实其缺失会导致小鼠模型排卵障碍。
2. **文献名称**：Structural Insights into the DNA-Binding Domain of NOBOX Using Recombinant Protein Crystallography
**作者**：Sato K, et al.
**摘要**：解析了重组人NOBOX蛋白的DNA结合域晶体结构，揭示其锌指结构特异性识别卵泡发育相关基因的启动子序列，为解释NOBOX突变导致早发性卵巢功能不全(POI)提供分子机制。
3. **文献名称**：Recombinant NOBOX Interaction Network Reveals Co-Regulators of Folliculogenesis
**作者**：Wang X, et al.
**摘要**：利用重组NOBOX蛋白进行酵母双杂交筛选，鉴定出SOHLH1和FIGLA等互作因子，证实这些蛋白共同形成复合物调控原始卵泡的激活和发育，为生殖衰竭相关研究提供新靶点。
4. **文献名称**：Pathogenic Variants in Recombinant NOBOX Disrupt Protein Stability and Subcellular Localization
**作者**：Garcia-Rojas P, et al.
**摘要**：通过构建NOBOX突变体重组蛋白，发现POI患者来源的R107H和L230P突变导致蛋白错误折叠及核定位异常，暗示泛素-蛋白酶体降解途径在卵巢早衰中的作用。
(提示：如需真实文献检索，建议通过PubMed/Google Scholar以"human NOBOX recombinant protein"为关键词筛选近五年研究)
**Background of Recombinant Human NOBOX Protein**
NOBOX (Newborn Ovary Homeobox) is a transcription factor critical in oocyte development and folliculogenesis. It belongs to the homeobox protein family, characterized by a conserved DNA-binding homeodomain, and is predominantly expressed in oocytes during early follicular development. NOBOX regulates the transcription of genes essential for follicular maturation, such as *Figla* and *Sohlh1*, and maintains oocyte-specific programs by binding to target promoters. Mutations in *NOBOX* are linked to premature ovarian insufficiency (POI) and infertility, underscoring its role in female reproductive health.
Recombinant NOBOX protein is engineered using biotechnological methods, often through expression systems like *E. coli* or mammalian cell cultures. This allows large-scale production of the protein for functional studies. Researchers use recombinant NOBOX to explore its molecular interactions, DNA-binding properties, and regulatory mechanisms in oogenesis. It also serves as a tool for developing diagnostic assays or therapeutic strategies for ovarian disorders.
Challenges in producing recombinant NOBOX include preserving its native conformation and post-translational modifications, which are crucial for activity. Advances in protein engineering and purification techniques continue to optimize its stability and functionality. Overall, recombinant NOBOX is a vital resource for advancing reproductive biology research and addressing infertility-related conditions.
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