| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOC4L |
| Uniprot No | Q9BVI4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-516 aa |
| 活性数据 | MEREPGAAGVRRALGRRLEAVLASRSEANAVFDILAVLQSEDQEEIQEAVRTCSRLFGALLERGELFVGQLPSEEMVMTGSQGATRKYKVWMRHRYHSCCNRLGELLGHPSFQVKELALSALLKFVQLEGAHPLEKSKWEGNYLFPRELFKLVVGGLLSPEEDQSLLLSQFREYLDYDDTRYHTMQAAVDAVARVTGQHPEVPPAFWNNAFTLLSAVSLPRREPTVSSFYVKRAELWDTWKVAHLKEHRRVFQAMWLSFLKHKLPLSLYKKVLLIVHDAILPQLAQPTLMIDFLTRACDLGGALSLLALNGLFILIHKHNLEYPDFYRKLYGLLDPSVFHVKYRARFFHLADLFLSSSHLPAYLVAAFAKRLARLALTAPPEALLMVLPFICNLLRRHPACRVLVHRPHGPELDADPYDPGEEDPAQSRALESSLWELQALQRHYHPEVSKAASVINQALSMPEVSIAPLLELTAYEIFERDLKKKGPEPVPLEFIPAQGLLGRPGELCAQHFTLS |
| 分子量 | 84.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOC4L蛋白的模拟参考文献示例，基于该领域的研究趋势整理而成。若需引用实际文献，请通过学术数据库核实：
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1. **文献名称**：*NOC4L interacts with NOC2L and NOC3L to mediate ribosomal RNA processing in human cells*
**作者**：T. Kudo, M. Fujiyama, et al.
**摘要**：该研究通过重组NOC4L蛋白体外实验，揭示其与NOC2L、NOC3L形成复合物，调控核糖体RNA前体(pre-rRNA)的切割和加工，对核糖体亚基成熟至关重要。
2. **文献名称**：*Depletion of NOC4L impairs cell proliferation through G1/S phase arrest*
**作者**：S. Müller, A. López-García, et al.
**摘要**：利用重组NOC4L蛋白的过表达/敲低模型，证明其通过影响Cyclin D1表达调控细胞周期，缺失导致G1/S期阻滞，提示其在增殖中的关键作用。
3. **文献名称**：*Crystal structure of the human NOC4L N-terminal domain reveals a conserved ribosome biogenesis motif*
**作者**：J. Zhang, R. Xu, et al.
**摘要**：解析重组NOC4L蛋白N端结构域晶体结构，发现其含有保守的核仁定位序列，并通过体外结合实验验证其与RNA解旋酶DDX21的相互作用。
4. **文献名称**：*NOC4L overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：L. Chen, Y. Wang, et al.
**摘要**：基于重组NOC4L蛋白的功能研究，发现其在肝癌中异常高表达，并通过PI3K/AKT通路促进肿瘤侵袭，为潜在治疗靶点提供依据。
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**注意事项**：
- 以上文献为示例性质，具体研究需以真实发表内容为准。
- 可通过PubMed、Google Scholar等平台检索关键词“NOC4L protein”或“NOC4L ribosome biogenesis”获取近期文献。
- NOC4L的研究多集中于核糖体合成、细胞周期及癌症关联领域。
NOC4L (Nucleolar Complex Associated 4 Homolog), previously known as PSO4 or C1ORF108. is a conserved eukaryotic protein primarily localized to the nucleolus, a subnuclear compartment critical for ribosome biogenesis. It plays a multifaceted role in cell cycle regulation, ribosome assembly, and genome stability maintenance. Structurally, NOC4L contains a conserved coiled-coil domain, facilitating protein-protein interactions. Studies indicate that NOC4L forms a complex with NOC2 and NOC3. functioning as a chaperone to mediate the export of pre-ribosomal particles from the nucleolus to the cytoplasm, ensuring proper ribosome maturation.
Beyond ribosome biogenesis, NOC4L is implicated in DNA damage response pathways. It interacts with BRCA1-BARD1. a key complex in DNA repair, suggesting a role in maintaining genomic integrity. Additionally, NOC4L contributes to cell cycle progression by regulating the G1/S transition checkpoint, with knockdown experiments leading to G1 phase arrest and apoptosis. Dysregulation of NOC4L has been linked to carcinogenesis. Its overexpression has been observed in certain cancers (e.g., hepatocellular carcinoma, colorectal cancer) and correlated with poor prognosis, potentially through pathways involving MYC oncogene activation or ribosomal stress responses.
Despite these findings, NOC4L's precise molecular mechanisms remain under investigation, particularly its interplay between ribosome assembly, cell cycle control, and tumorigenesis. Evolutionary conservation across species underscores its fundamental biological significance.
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