| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOL14 |
| Uniprot No | P78316 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-806 aa |
| 活性数据 | MAKAKKVGARRKASGAPAGARGGPAKANSNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARALRKRTQTLLKEYKERDKSNVFRDKRFGEYNSNMSPEEKMMKRFALEQQRHHEKKSIYNLNEDEELTHYGQSLADIEKHNDIVDSDSDAEDRGTLSAELTAAHFGGGGGLLHKKTQQEGEEREKPKSRKELIEELIAKSKQEKRERQAQREDALELTEKLDQDWKEIQTLLSHKTPKSENRDKKEKPKPDAYDMMVRELGFEMKAQPSNRMKTEAELAKEEQEHLRKLEAERLRRMLGKDEDENVKKPKHMSADDLNDGFVLDKDDRRLLSYKDGKMNVEEDVQEEQSKEASDPESNEEEGDSSGGEDTEESDSPDSHLDLESNVESEEENEKPAKEQRQTPGKGLISGKERAGKATRDELPYTFAAPESYEELRSLLLGRSMEEQLLVVERIQKCNHPSLAEGNKAKLEKLFGFLLEYVGDLATDDPPDLTVIDKLVVHLYHLCQMFPESASDAIKFVLRDAMHEMEEMIETKGRAALPGLDVLIYLKITGLLFPTSDFWHPVVTPALVCLSQLLTKCPILSLQDVVKGLFVCCLFLEYVALSQRFIPELINFLLGILYIATPNKASQGSTLVHPFRALGKNSELLVVSAREDVATWQQSSLSLRWASRLRAPTSTEANHIRLSCLAVGLALLKRCVLMYGSLPSFHAIMGPLQALLTDHLADCSHPQELQELCQSTLTEMESQKQLCRPLTCEKSKPVPLKLFTPRLVSNSRPQVIHLPWPPNYRCEPQHPAKKVIFFRVIML |
| 分子量 | 117.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的重组人NOL14蛋白相关参考文献示例(具体文献需通过学术数据库验证)：
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1. **文献名称**: "Recombinant expression and functional analysis of human NOL14 in rRNA processing"
**作者**: Smith A, et al.
**摘要**: 报道了在大肠杆菌系统中重组表达人NOL14蛋白的方法，纯化后通过体外实验证实其参与pre-rRNA的切割，并发现与核糖体组装因子相互作用。
2. **文献名称**: "Structural insights into NOL14's role in ribosome biogenesis"
**作者**: Zhang Y, et al.
**摘要**: 利用X射线晶体学解析了重组人NOL14蛋白的C端结构域，揭示其与RNA结合的分子机制，提出其作为核仁复合物支架的功能模型。
3. **文献名称**: "NOL14 interacts with viral RNA to modulate host-cell responses"
**作者**: Lee H, et al.
**摘要**: 研究重组NOL14蛋白与RNA病毒的相互作用，发现其通过结合病毒基因组RNA抑制复制，为抗病毒药物开发提供潜在靶点。
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**注意**：以上文献为示例性质，实际研究中建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "recombinant human NOL14" 或 "NOL14 protein function" 获取真实文献。NOL14的别名可能包括C14orf166.建议同时检索别名以提高查全率。
**Background of Recombinant Human NOL14 Protein**
NOL14 (Nucleolar Protein 14), also known as C1orf87. is a conserved eukaryotic protein primarily localized in the nucleolus, a subcellular compartment critical for ribosome biogenesis. It plays a role in pre-rRNA processing and ribosome assembly, contributing to nucleolar organization and RNA metabolism. Human NOL14 contains a PIN (PilT N-terminus)-like domain, suggesting potential involvement in nucleic acid binding or enzymatic activities. Studies link NOL14 to cell cycle regulation, with its dysregulation implicated in diseases such as myelodysplastic syndromes and acute myeloid leukemia.
Recombinant NOL14 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional and structural studies. Its production allows researchers to investigate interactions with binding partners like PIN1 and its role in ribosomal RNA maturation. Recombinant versions are essential for *in vitro* assays, structural analysis, and screening for therapeutic agents targeting nucleolar dysfunction. The protein’s molecular weight (~55 kDa) and domain architecture make it a subject of interest in understanding ribosomopathies and cancer mechanisms. Ongoing research aims to clarify its precise molecular functions and therapeutic potential in diseases linked to ribosomal stress.
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