| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OGFOD1 |
| Uniprot No | Q8N543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542 aa |
| 活性数据 | MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS HEVIVMDMDP FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ SDDLKKRREP HISTLRKILF EDFRSWLSDI SKIDLESTID MSCAKYEFTD ALLCHDDELE GRRIAFILYL VPPWDRSMGG TLDLYSIDEH FQPKQIVKSL IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP SLTRPPNYFE PPIPRSPHIP QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK PEKFTKVCEA LEHGHVEWSS RGPPNKRFYE KAEESKLPEI LKECMKLFRS EALFLLLSNF TGLKLHFLAP SEEDEMNDKK EAETTDITEE GTSHSPPEPE NNQMAISNNS QQSNEQTDPE PEENETKKES SVPMCQGELR HWKTGHYTLI HDHSKAEFAL DLILYCGCEG WEPEYGGFTS YIAKGEDEEL LTVNPESNSL ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE |
| 分子量 | 63.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OGFOD1蛋白研究的3篇示例参考文献(内容为虚构示例，仅作格式参考)：
1. **《OGFOD1 regulates ribosomal translation elongation via Pro-62 hydroxylation in stress conditions》**
- **作者**: Smith J, et al.
- **摘要**: 研究揭示了OGFOD1作为脯氨酸羟化酶的功能，可在细胞应激条件下羟化核糖体蛋白S23的第62位脯氨酸，调控翻译暂停及应激颗粒形成，影响细胞存活。
2. **《Structural and functional analysis of human OGFOD1 in hypoxia signaling》**
- **作者**: Yamamoto T, et al.
- **摘要**: 通过晶体结构解析发现，OGFOD1依赖2-酮戊二酸和铁离子催化底物羟化，并证实其与HIF-1α通路存在交叉调控，暗示其在缺氧适应中的潜在作用。
3. **《OGFOD1 knockout mice exhibit neurological defects and altered mRNA translation》**
- **作者**: Schmidt R, et al.
- **摘要**: 构建OGFOD1基因敲除小鼠模型，发现其神经发育异常与核糖体翻译速率下降相关，提示OGFOD1在神经系统中的关键调控功能。
**注**：以上文献为示例，实际研究需通过PubMed、Web of Science等数据库检索真实论文。如需具体文献，建议使用关键词“OGFOD1”“hydroxylase”“ribosomal translation”等筛选近年高影响力研究。
OGFOD1 (2-Oxoglutarate and Iron-Dependent Oxygenase Domain-Containing Protein 1) is a member of the 2-oxoglutarate (2-OG) and Fe²⁺-dependent dioxygenase family, which plays a role in post-translational modifications and cellular stress responses. It catalyzes the hydroxylation of specific proline residues in target proteins, a process dependent on 2-OG, molecular oxygen, and Fe²⁺. One well-characterized substrate is the ribosomal protein RPS23. where OGFOD1-mediated proline hydroxylation influences translation fidelity and stress adaptation.
Functionally, OGFOD1 is implicated in regulating cellular processes under hypoxic conditions, DNA damage repair, and cell cycle progression. Studies link its activity to pathways involving hypoxia-inducible factors (HIFs), though it operates independently of canonical HIF signaling. Dysregulation of OGFOD1 has been associated with cancer, ischemic diseases, and neurological disorders, suggesting its potential as a therapeutic target.
Recombinant human OGFOD1 protein, produced via expression systems like *E. coli* or mammalian cells, retains enzymatic activity and is widely used *in vitro* to study hydroxylation mechanisms, screen inhibitors, or explore protein interactions. Its structural features, including conserved oxygenase and substrate-binding domains, enable investigations into enzyme kinetics and substrate specificity. Research on OGFOD1 continues to uncover its role in cellular stress adaptation and disease pathology, highlighting its significance in both basic and translational biomedical studies.
×
