| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ODF2 |
| Uniprot No | Q14990 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250 aa |
| 活性数据 | MAALSCLLDS VRRDIKKVDR ELRQLRCIDE FSTRCLCDLY MHPYCCCDLH PYPYCLCYSK RSRSCGLCDL YPCCLCDYKL YCLRPSLRSL ERKAIRAIED EKRELAKLRR TTNRILASSC CSSNILGSVN VCGFEPDQVK VRVKDGKVCV SAERENRYDC LGSKKYSYMN ICKEFSLPPC VDEKDVTYSY GLGSCVKIES PCYPCTSPCS PCSPCSPCNP CSPCNPCSPY DPCNPCYPCG SRFSCRKMIL |
| 分子量 | 28.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ODF2蛋白的参考文献示例(*注：因无法实时查询数据库，以下内容为基于领域知识的模拟示例，建议通过学术引擎验证并获取真实文献*)：
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1. **文献名称**: **"Recombinant human ODF2 facilitates centriole amplification in vitro via microtubule stabilization"**
**作者**: Nakamura, T., et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人ODF2蛋白，发现其通过稳定微管结构促进体外中心体扩增，揭示了ODF2在有丝分裂及纤毛形成中的关键作用。
2. **文献名称**: **"Structural characterization of ODF2’s coiled-coil domains and their role in sperm tail assembly"**
**作者**: Kumar, S., Smith, J.
**摘要**: 通过冷冻电镜解析重组ODF2蛋白的卷曲螺旋结构域，阐明其在精子尾部致密纤维形成中的组装机制，为男性不育症的分子机制提供新见解。
3. **文献名称**: **"ODF2 interacts with CEP128 to regulate ciliogenesis: Insights from recombinant protein binding assays"**
**作者**: Li, Y., et al.
**摘要**: 利用重组ODF2与CEP128进行体外结合实验，证明两者相互作用调控纤毛发生，并发现突变体ODF2可导致纤毛缺陷相关疾病(如多囊肾)。
4. **文献名称**: **"Development of an ODF2-based biomarker assay for cancer diagnosis using recombinant protein technology"**
**作者**: Wang, H., et al.
**摘要**: 基于重组ODF2蛋白开发高灵敏度检测方法，证实其在多种癌症患者血清中异常高表达，提示ODF2作为肿瘤标志物的潜力。
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**建议**：使用PubMed或Google Scholar搜索关键词“**recombinant ODF2**”或“**ODF2 protein function**”，筛选近5年文献以获取最新进展。
Recombinant human ODF2 (Outer Dense Fiber Protein 2), also known as Cenexin, is a structural protein primarily associated with spermatogenesis and sperm function. It is a key component of the outer dense fibers in the sperm tail, contributing to its mechanical stability and motility. ODF2 is characterized by multiple coiled-coil domains, which mediate protein-protein interactions critical for sperm tail assembly. Beyond germ cells, ODF2 plays roles in somatic cells, including centriole maturation, ciliogenesis, and cell cycle regulation, linking it to broader cellular processes like mitosis and cytoskeletal organization.
Produced through recombinant DNA technology, ODF2 is expressed in host systems (e.g., bacteria, mammalian cells) to generate purified protein for functional studies. This recombinant form enables research into its molecular interactions, structural contributions to sperm architecture, and involvement in cellular mechanisms such as centrosome integrity and ciliary biogenesis. Studies using recombinant ODF2 have identified its binding partners and regulatory pathways, offering insights into male infertility caused by sperm structural defects. Additionally, due to its role in centriole function, ODF2 is explored in contexts like cancer, where centrosomal abnormalities may drive genomic instability.
The protein’s recombinant version also aids in developing diagnostic tools or therapeutic strategies targeting ODF2-related disorders, highlighting its dual significance in reproductive health and somatic disease research.
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