关键信息
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基因名
c-Kit
- 应用
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别名
c-Kit；SCFR；Mast/stem cell growth factor receptor Kit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10721-2
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表达区间
26-516aa
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氨基酸序列
QPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETN ENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLY GKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSV KRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLRE GEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATL TISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFV NDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVS ELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGM LQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQ SSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKEQIHPHT
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分子量
82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
c-Kit, also known as CD117, is a receptor tyrosine kinase encoded by the KIT gene, which plays a crucial role in cell signaling, particularly in hematopoiesis, development of germ cells, and maintenance of the interstitial cells of Cajal in the gastrointestinal tract. Mutations and aberrant activation of c-Kit are implicated in various cancers, including mastocytosis and certain gastrointestinal stromal tumors (GISTs), making it a significant target for therapeutic intervention. The study of c-Kit recombinant proteins has emerged as a pivotal area of research, focusing on their structure, function, and potential as biomarkers or therapeutic targets. Recombinant c-Kit proteins allow for the investigation of its molecular mechanisms and interactions in a controlled setting, facilitating drug development aimed at inhibiting its overactivity. Advances in protein engineering and expression systems have enabled the production of functional c-Kit proteins, paving the way for high-throughput screening of small molecules as potential inhibitors. Moreover, understanding the signaling pathways activated by c-Kit can provide insights into its role in tumorigenesis and resistance to existing therapies, highlighting the importance of c-Kit research in oncology and regenerative medicine. This line of research holds the promise of discovering novel therapeutic strategies that could enhance treatment outcomes for patients with c-Kit-related malignancies.
