| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4N5 |
| Uniprot No | Q8IXE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308 aa |
| 活性数据 | METQNLTVVTEFILLGLTQSQDAQLLVFVLVLIFYLIILPGNFLIIFTIKSDPGLTAPLY FFLGNLALLDASYSFIVVPRMLVDFLSEKKVISYRSCITQLFFLHFLGAGEMFLLVVMAF DRYIAICRPLHYSTIMNPRACYALSLVLWLGGFIHSIVQVALILHLPFCGPNQLDNFFCD VPQVIKLACTNTFVVELLMVSNSGLLSLLCFLGLLASYAVILCRIREHSSEGKSKAISTC TTHIIIIFLMFGPAIFIYTCPFQAFPADKVVSLFHTVIFPLMNPVIYTLRNQEVKASMRK LLSQHMFC |
| 分子量 | 34.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4N5蛋白的3篇参考文献示例，涵盖功能、表达技术及疾病相关性研究：
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1. **"Functional characterization of the human olfactory receptor OR4N5"**
*Authors: Smith J, et al. (2022)*
**摘要**: 本研究在HEK293细胞中重组表达OR4N5蛋白，通过钙成像技术筛选其配体，发现该受体对特定挥发性醛类化合物有响应，揭示了其在嗅觉感知中的潜在作用。
2. **"Heterologous expression and purification of human OR4N5 in insect cells"**
*Authors: Lee S, Kim M (2020)*
**摘要**: 开发了一种基于杆状病毒-昆虫细胞系统的OR4N5重组表达方法，获得高纯度蛋白用于结构研究，为解析该受体的三维构象提供技术基础。
3. **"OR4N5 overexpression correlates with metabolic syndrome progression"**
*Authors: Zhang R, et al. (2021)*
**摘要**: 通过分析代谢综合征患者的脂肪组织样本，发现OR4N5蛋白异常高表达，提示其可能参与脂质代谢调控，拓展了嗅觉受体的非嗅觉功能认知。
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**说明**：
- 以上文献为模拟示例，实际研究可能需查阅PubMed或SciFinder。若需具体文献，建议通过关键词“OR4N5 recombinant protein”在学术数据库检索。
- OR4N5研究相对有限，可扩展至嗅觉受体家族(如OR1A1/OR2T1等)表达技术或功能研究作为补充。
**Background of Recombinant Human OR4N5 Protein**
The recombinant human OR4N5 protein is a synthesized form of the olfactory receptor OR4N5. a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are primarily responsible for detecting odorants in the nasal epithelium, but emerging studies suggest their expression in non-olfactory tissues, implying broader physiological roles. OR4N5. encoded by the *OR4N5* gene, is classified under subfamily 4N within the OR family. While its exact biological function remains unclear, it is hypothesized to participate in chemosensory signaling or potential non-olfactory processes, such as cellular regulation or disease pathways.
Recombinant OR4N5 is produced using biotechnological platforms (e.g., bacterial, insect, or mammalian cell systems), enabling precise control over protein purity and post-translational modifications. This engineered protein facilitates structural and functional studies, including ligand-binding assays, receptor activation mechanisms, and interaction networks. Its applications span basic research—deciphering OR signaling pathways—and drug discovery, particularly in targeting GPCR-related disorders. Challenges include optimizing expression systems due to ORs’ complex transmembrane structures and low native expression levels. Current research focuses on identifying OR4N5-specific ligands and elucidating its role in human health, potentially unlocking therapeutic avenues for conditions linked to olfactory or ectopic receptor dysregulation.
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