| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4K14 |
| Uniprot No | Q8NGD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310 aa |
| 活性数据 | MDPQNYSLVSEFVLHGLCTSRHLQNFFFIFFFGVYVAIMLGNLLILVTVISDPCLHSSPM YFLLGNLAFLDMWLASFATPKMIRDFLSDQKLISFGGCMAQIFFLHFTGGAEMVLLVSMA YDRYVAICKPLHYMTLMSWQTCIRLVLASWVVGFVHSISQVAFTVNLPYCGPNEVDSFFC DLPLVIKLACMDTYVLGIIMISDSGLLSLSCFLLLLISYTVILLAIRQRAAGSTSKALST CSAHIMVVTLFFGPCIFVYVRPFSRFSVDKLLSVFYTIFTPLLNPIIYTLRNEEMKAAMK KLQNRRVTFQ |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的关于重组人OR4K14蛋白的参考文献，仅供参考(实际文献需根据具体研究查询验证)：
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1. **文献名称**：*"Functional expression and ligand identification of the human olfactory receptor OR4K14 in a heterologous system"*
**作者**：Zhang, L. et al.
**摘要**：研究成功在HEK293细胞中重组表达了OR4K14蛋白，并通过基于cAMP的报告基因系统筛选其配体，发现该受体对庚醛等中链醛类化合物有特异性响应，提示其在嗅觉感知中的潜在作用。
2. **文献名称**：*"Structural insights into the olfactory receptor OR4K14 through recombinant protein purification and molecular dynamics simulations"*
**作者**：Vargas, R. et al.
**摘要**：利用杆状病毒-昆虫细胞系统纯化重组OR4K14蛋白，结合分子动力学模拟揭示了其跨膜结构域的构象变化，阐明了其配体结合的动态机制。
3. **文献名称**：*"OR4K14 beyond olfaction: Recombinant expression reveals a role in hepatic lipid metabolism"*
**作者**：Kim, S. et al.
**摘要**：在肝细胞系中异源表达OR4K14.发现其与脂肪酸代谢相关基因的表达调控有关，推测该受体可能通过感知内源性脂类分子参与代谢疾病病理过程。
4. **文献名称**：*"Development of a nanobody-based biosensor for detecting OR4K14 activation"*
**作者**：Müller, P. et al.
**摘要**：通过重组OR4K14蛋白与特异性纳米抗体结合，构建了实时监测受体激活的荧光生物传感器，为高通量药物筛选提供了新工具。
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**注**：以上文献为假设性示例，若需真实文献，建议在PubMed、Google Scholar等平台以“OR4K14 recombinant protein”或“olfactory receptor OR4K14 expression”为关键词检索。实际研究中，OR4K14的相关文献可能较少，可扩展至嗅觉受体重组表达的技术类文献作为参考。
Recombinant human OR4K14 protein is a genetically engineered form of the olfactory receptor 4K14. a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs), encoded by the largest gene family in the human genome, are primarily responsible for detecting odorant molecules and mediating signal transduction in olfactory sensory neurons. OR4K14 is classified as a pseudogene in some populations due to frameshift mutations or premature stop codons, suggesting potential loss of functional expression in humans. However, recombinant production enables biochemical and structural studies to explore its hypothetical role or evolutionary divergence.
Expressed in heterologous systems like *E. coli* or mammalian cells, recombinant OR4K14 is often purified using affinity tags for experimental applications. Research focuses on its ligand-binding potential, cellular localization, or interactions with olfactory signaling components. While its physiological relevance remains unclear, studying OR4K14 may contribute to understanding pseudogene functionalization, olfactory receptor evolution, or compensatory mechanisms in嗅觉功能障碍. Challenges include achieving proper membrane folding and identifying activators due to ORs' inherent hydrophobicity. Overall, recombinant OR4K14 serves as a tool to probe the enigmatic biology of non-functional ORs and their contextual roles beyond olfaction.
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