| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4K13 |
| Uniprot No | Q8NH42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304 aa |
| 活性数据 | MERANHSVVSEFILLGLSKSQNLQILFFLGFSVVFVGIVLGNLLILVTVTFDSLLHTPMY FLLSNLSCIDMILASFATPKMIVDFLRERKTISWWGCYSQMFFMHLLGGSEMMLLVAMAI DRYVAICKPLHYMTIMSPRVLTGLLLSSYAVGFVHSSSQMAFMLTLPFCGPNVIDSFFCD LPLVIKLACKDTYILQLLVIADSGLLSLVCFLLLLVSYGVIIFSVRYRAASRSSKAFSTL SAHITVVTLFFAPCVFIYVWPFSRYSVDKILSVFYTIFTPLLNPIIYTLRNQEVKAAIKK RLCI |
| 分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人OR4K13蛋白相关的模拟参考文献示例(需注意：OR4K13研究较少，部分内容基于同类型蛋白研究推断，建议结合实际文献库验证)：
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1. **文献名称**: *Heterologous Expression and Ligand Screening of Human Olfactory Receptor OR4K13 in HEK293 Cells*
**作者**: Smith J, et al.
**摘要**: 本研究成功在HEK293细胞中表达重组OR4K13蛋白，并通过荧光报告系统筛选潜在配体，发现其对特定短链醛类化合物表现出响应，为嗅觉受体功能研究提供模型。
2. **文献名称**: *Structural Insights into OR4K13 via Cryo-EM: Implications for Odorant Recognition*
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜技术首次解析OR4K13的三维结构，揭示其跨膜域中保守的配体结合口袋，为理解嗅觉受体特异性识别机制提供结构基础。
3. **文献名称**: *Optimization of Recombinant OR4K13 Production in Baculovirus-Insect Cell Systems*
**作者**: Müller R, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统优化OR4K13表达条件，提高蛋白产量及稳定性，并验证其与G蛋白偶联活性，为高通量筛选奠定技术基础。
4. **文献名称**: *Functional Characterization of OR4K13 Polymorphisms in Human Populations*
**作者**: García-Sánchez A, et al.
**摘要**: 分析OR4K13基因多态性对重组蛋白功能的影响，发现某些突变体对特定气味分子敏感性下降，提示遗传变异可能影响个体嗅觉差异。
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**注意**：以上文献为示例性质，实际研究中OR4K13相关文献较少，建议通过以下方式扩展检索：
- 使用数据库(PubMed/Google Scholar)以 **"OR4K13 recombinant"、"olfactory receptor OR4K13 expression"** 等关键词搜索;
- 查阅**嗅觉受体异源表达**或**G蛋白偶联受体重组技术**的相关综述(如 *Frontiers in Molecular Biosciences* 中的方法论文章)。
**Background of Recombinant Human OR4K13 Protein**
The recombinant human OR4K13 protein is a genetically engineered form of the olfactory receptor OR4K13. a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are crucial for detecting odorants and mediating olfactory signal transduction. OR4K13. encoded by the *OR4K13* gene located on chromosome 14. is classified under the olfactory receptor family 4 subfamily K. While the exact ligands and physiological roles of OR4K13 remain poorly characterized, it is hypothesized to participate in odorant recognition, contributing to the diversity of human olfactory perception.
Recombinant OR4K13 is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable detailed structural and functional studies. These systems often incorporate affinity tags (e.g., His-tag) for purification and detection. Research on recombinant OR4K13 aids in elucidating its ligand-binding specificity, activation mechanisms, and downstream signaling pathways, which are challenging to study in native tissues due to low receptor abundance and tissue-specific expression.
Despite its classification as a pseudogene in some databases, emerging evidence suggests potential low-level expression or functional relevance in non-olfactory tissues, sparking interest in its roles beyond smell, such as in disease mechanisms or cellular signaling. Recombinant OR4K13 serves as a tool for drug discovery, sensory biology research, and the development of biosensors. However, further studies are needed to validate its functional significance and explore therapeutic applications.
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