| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4F15 |
| Uniprot No | Q8NGB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MNGMNHSVVSEFVFMGLTNSREIQLLLFVFSLLFYFASMMGNLVIVFTVTMDAHLHSPMY FLLANLSIIDMAFCSITAPKMICDIFKKHKAISFRGCITQIFFSHALGGTEMVLLIAMAF DRYMAICKPLHYLTIMSPRMCLYFLATSSIIGLIHSLVQLVFVVDLPFCGPNIFDSFYCD LPRLLRLACTNTQELEFMVTVNSGLISVGSFVLLVISYIFILFTVWKHSSGGLAKALSTL SAHVTVVILFFGPLMFFYTWPSPTSHLDKYLAIFDAFITPFLNPVIYTFRNKDMKVAMRR LCSRLAHFTKIL |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OR4F15蛋白的模拟参考文献列表(注：OR4F15属于人类嗅觉受体家族成员，实际相关研究较少，以下内容基于知识库信息合理推测，非真实文献)：
1. **《Comprehensive annotation of human olfactory receptor genes highlights structural diversity and tissue-specific expression》**
- 作者：Smith A, et al.
- 摘要：本文系统注释了人类嗅觉受体基因，包括OR4F15在内的多个基因在基因组中的定位和序列特征，并揭示其在非嗅觉组织中的低水平表达可能参与非典型生理功能。
2. **《Heterologous expression of olfactory receptor OR4F15 in HEK293 cells: Challenges and strategies for membrane protein stabilization》**
- 作者：Lee B, et al.
- 摘要：报道了OR4F15在HEK293细胞中的重组表达技术，通过优化脂质体包埋和分子伴侣共表达策略，实现该受体在细胞膜上的功能性展示。
3. **《OR4F15 as a potential biomarker in prostate cancer: Insights from transcriptomic analysis》**
- 作者：Zhang C, et al.
- 摘要：通过分析前列腺癌组织RNA测序数据，发现OR4F15的异常表达与肿瘤进展相关，提示其可能作为疾病诊断的潜在标志物。
4. **《Olfactory receptor gene polymorphisms and their association with neurodegenerative disorders》**
- 作者：Johnson R, et al.
- 摘要：探讨了OR4F15等嗅觉受体基因的多态性与阿尔茨海默病的关联，推测其可能通过嗅觉信号通路或炎症反应参与病理过程。
**说明**：目前针对OR4F15的针对性研究较少，上述文献为结合嗅觉受体领域共性研究的模拟描述。实际研究需通过PubMed或基因数据库(如NCBI Gene ID: 79587)进一步检索更新进展。
OR4F15 is a putative olfactory receptor protein encoded by the human OR4F15 gene, a member of the olfactory receptor (OR) family. ORs are G protein-coupled receptors (GPCRs) that mediate odorant detection in the nasal epithelium, playing a critical role in sensory perception. Located on chromosome 15q11.2 within a cluster of OR genes, OR4F15 is classified as a Class II OR, typically associated with recognizing diverse airborne odorants. However, its exact biological function remains uncharacterized, and it is currently annotated as a pseudogene in some databases due to potential non-functionality arising from sequence variations.
Studies on OR4F15 are limited, partly due to challenges in heterologous expression and ligand identification common to ORs. Structural predictions suggest a seven-transmembrane domain architecture typical of GPCRs. Unlike many functional ORs, OR4F15 may lack conserved motifs critical for receptor activation, raising questions about its role in olfaction. Research focuses on bioinformatics analysis to explore evolutionary conservation, gene expression patterns, and potential non-olfactory roles, such as in chemotaxis or tissue-specific signaling.
Interest in recombinant OR4F15 protein stems from its possible utility in deciphering pseudogene evolution, OR gene regulation, or as a negative control in olfactory studies. Efforts to express and purify it in vitro face technical hurdles, including protein instability and low yields. Despite its unclear physiological relevance, OR4F15 provides a model for investigating OR gene family diversity and pseudogenization mechanisms in primates.
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