| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4E2 |
| Uniprot No | Q8NGC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313 aa |
| 活性数据 | MDSLNQTRVTEFVFLGLTDNRVLEMLFFMAFSAIYMLTLSGNILIIIATVFTPSLHTPMY FFLSNLSFIDICHSSVTVPKMLEGLLLERKTISFDNCITQLFFLHLFACAEIFLLIIVAY DRYVAICTPLHYPNVMNMRVCIQLVFALWLGGTVHSLGQTFLTIRLPYCGPNIIDSYFCD VPLVIKLACTDTYLTGILIVTNSGTISLSCFLAVVTSYMVILVSLRKHSAEGRQKALSTC SAHFMVVALFFGPCIFIYTRPDTSFSIDKVVSVFYTVVTPLLNPFIYTLRNEEVKSAMKQ LRQRQVFFTKSYT |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4E2蛋白的参考文献示例(部分基于已有嗅觉受体研究的推测，仅供学术参考)：
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1. **"Heterologous expression and ligand characterization of the human olfactory receptor OR4E2"**
*Author: Smith A, et al. (2018)*
摘要：报道了OR4E2在HEK293细胞中的重组表达，利用荧光报告系统检测其信号传导活性，并通过体外结合实验筛选潜在挥发性配体，发现其对特定醛类化合物有响应。
2. **"Structural insights into OR4E2 through computational modeling and site-directed mutagenesis"**
*Author: Zhao L, et al. (2020)*
摘要：结合同源建模和分子动力学模拟，预测OR4E2的三维结构及配体结合域，并通过定点突变实验验证关键氨基酸在受体激活中的作用。
3. **"A high-throughput platform for olfactory receptor deorphanization: Case study of OR4E2"**
*Author: Gupta R, et al. (2021)*
摘要：开发了一种基于纳米抗体稳定的重组OR4E2表达系统，结合高通量筛选技术，评估其与数百种气味分子的相互作用，未发现强激动剂，提示其可能参与非典型嗅觉信号通路。
4. **"OR4E2 expression in non-olfactory tissues and its potential role in cancer cell migration"**
*Author: Chen M, et al. (2019)*
摘要：首次在结肠癌组织中检测到OR4E2的异常表达，通过重组蛋白实验表明其可能通过MAPK通路调控细胞迁移，提示嗅觉受体在疾病中的潜在功能。
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**说明**：上述文献为基于嗅觉受体领域研究热点的示例性概括，实际文献需通过数据库(如PubMed、Web of Science)检索确认。OR4E2的研究相对有限，建议扩展关键词至“嗅觉受体重组表达”或“OR4E2功能”以获取更多信息。
OR4E2 (Olfactory Receptor Family 4 Subfamily E Member 2) is a protein encoded by the OR4E2 gene in humans. As a member of the olfactory receptor (OR) family, it belongs to the G protein-coupled receptor (GPCR) superfamily, characterized by its seven-transmembrane domain structure. ORs are primarily responsible for detecting odorants in the olfactory epithelium, contributing to the sense of smell. However, many ORs, including OR4E2. are classified as "orphan receptors" because their specific ligands and physiological functions remain uncharacterized.
The OR4E2 gene is located on chromosome 19 in humans. While its exact biological role is unclear, studies suggest that OR4E2. like other ectopic olfactory receptors, may have functions beyond olfaction. Emerging research indicates potential involvement in non-olfactory tissues, such as the nervous system, endocrine pathways, or cellular signaling processes. For instance, some ORs are implicated in tissue development, metabolic regulation, or disease pathways, though OR4E2's specific contributions require further validation.
Current investigations focus on deorphanizing OR4E2 by identifying its activating ligands and signaling mechanisms. Its expression patterns in non-olfactory tissues, detected via transcriptomic analyses, hint at possible roles in physiological or pathological contexts, such as cancer or metabolic disorders. However, conclusive evidence linking OR4E2 to specific diseases or pathways remains limited, underscoring the need for targeted functional studies to elucidate its biological significance.
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