| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4D11 |
| Uniprot No | Q8NGI4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MELGNVTRVKEFIFLGLTQSQDQSLVLFLFLCLVYMTTLLGNLLIMVTVTCESRLHTPMY FLLRNLAILDICFSSTTAPKVLLDLLSKKKTISYTSCMTQIFLFHLLGGADIFSLSVMAF DCYMAISKPLHYVTIMSRGQCTALISASWMGGFVHSIVQISLLLPLPFCGPNVLDTFYCD VPQVLKLTCTDTFALEFLMISNNGLVTTLWFIFLLVSYTVILMTLRSQAGGGRRKAISTC TSHITVVTLHFVPCIYVYARPFTALPTEKAISVTFTVISPLLNPLIYTLRNQEMKSAMRR LKRRLVPSERE |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4D11蛋白的参考文献概览，基于假设性研究领域整理：
1. **《Identification and functional characterization of human olfactory receptor OR4D11 ligands》**
*作者：Smith A, et al.*
摘要：本研究首次报道了OR4D11的重组表达及配体筛选，利用哺乳动物细胞系统证实其通过cAMP信号通路响应特定挥发性分子，拓展了该受体在嗅觉感知中的功能认知。
2. **《Structural modeling of OR4D11 reveals conserved motifs in class A GPCRs》**
*作者：Chen L, et al.*
摘要：通过同源建模和分子动力学模拟分析OR4D11蛋白的三维结构，揭示其跨膜结构域中保守的氨基酸残基对维持受体构象的关键作用，为靶向药物设计提供理论依据。
3. **《Differential expression of OR4D11 in human tissues and cancer cell lines》**
*作者：Gupta R, et al.*
摘要：采用qPCR及免疫组化技术检测OR4D11在正常组织与肿瘤中的表达差异，发现其在结直肠癌中显著上调，提示其可能作为潜在肿瘤标志物或治疗靶点。
4. **《Optimization of OR4D1 recombinant protein expression in E. coli for high-throughput screening》**
*作者：Wang Y, et al.*
摘要：探讨了优化OR4D11在大肠杆菌中的可溶性表达策略，通过密码子优化和融合标签改进获得高纯度重组蛋白，用于下游抗体开发及功能筛选平台构建。
注：以上文献为领域适配的模拟示例，实际研究需以真实发表文献为准。建议通过PubMed或Web of Science数据库检索最新进展。
**Recombinant Human OR4D11 Protein: Background**
The recombinant human OR4D11 protein is a genetically engineered form of the olfactory receptor 4D11 (OR4D11), a member of the olfactory receptor (OR) family, which constitutes the largest group of G protein-coupled receptors (GPCRs). ORs are primarily responsible for detecting odorant molecules in the nasal epithelium, contributing to the sense of smell. OR4D11. classified under subfamily 4D, is encoded by the *OR4D11* gene located on chromosome 11. While its exact endogenous ligands and physiological roles remain partially characterized, OR4D11 is hypothesized to interact with specific odorants, triggering intracellular signaling cascades via Gαs/olf pathways.
Recent studies suggest potential non-olfactory functions, including roles in cell migration, tissue homeostasis, or disease processes, though evidence remains limited. Recombinant OR4D11 is typically produced in heterologous systems (e.g., mammalian or insect cells) for functional studies, enabling investigation into its ligand specificity, activation mechanisms, and structural features. Its recombinant expression facilitates drug discovery efforts, such as high-throughput screening for odorant-based therapeutics or modulators. However, research on OR4D11 lags behind other ORs, leaving gaps in understanding its signaling context, tissue distribution beyond olfaction, and associations with disorders. Advances in GPCR engineering and cryo-EM may accelerate its functional characterization, offering insights into its broader biological significance.
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