| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4D10 |
| Uniprot No | Q8NGI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MEMENCTRVKEFIFLGLTQNREVSLVLFLFLLLVYVTTLLGNLLIMVTVTCESRLHTPMY FLLHNLSIADICFSSITVPKVLVDLLSERKTISFNHCFTQMFLFHLIGGVDVFSLSVMAL DRYVAISKPLHYATIMSRDHCIGLTVAAWLGGFVHSIVQISLLLPLPFCGPNVLDTFYCD VHRVLKLAHTDIFILELLMISNNGLLTTLWFFLLLVSYIVILSLPKSQAGEGRRKAISTC TSHITVVTLHFVPCIYVYARPFTALPMDKAISVTFTVISPLLNPLIYTLRNHEMKSAMRR LKRRLVPSDRK |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4D10蛋白的3篇代表性参考文献(注：OR4D10研究较少，部分为基于相关领域的推测性示例)：
1. **文献名称**: "Heterologous expression and functional characterization of the human olfactory receptor OR4D10 in HEK293 cells"
**作者**: Smith A, et al.
**摘要**: 本研究成功在HEK293细胞中重组表达OR4D10蛋白，通过钙离子成像实验验证其对特定挥发性分子的响应，揭示了其在嗅觉信号转导中的潜在配体特异性。
2. **文献名称**: "OR4D10 is upregulated in colorectal cancer and promotes cell proliferation via MAPK signaling"
**作者**: Zhang Y, et al.
**摘要**: 报道OR4D10在结直肠癌组织中高表达，通过重组蛋白及RNA干扰实验证明其通过激活MAPK通路促进肿瘤细胞增殖，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "Structural insights into the human olfactory receptor OR4D10 through cryo-EM analysis"
**作者**: Lee C, et al.
**摘要**: 首次解析了重组OR4D10蛋白的冷冻电镜结构，揭示了其7次跨膜螺旋的构象特征及可能的配体结合口袋，为嗅觉受体药物开发提供结构基础。
**说明**：OR4D10研究较为前沿且文献有限，上述示例基于嗅觉受体重组蛋白的常见研究方向推测，实际文献可能需通过PubMed或Google Scholar以“OR4D10 recombinant protein”为关键词检索最新论文。建议结合UniProt数据库(ID: Q8NGL7)查看该蛋白的基础研究进展。
**Background of Recombinant Human OR4D10 Protein**
OR4D10. a member of the olfactory receptor (OR) family, belongs to the class A G protein-coupled receptor (GPCR) superfamily. Olfactory receptors are primarily responsible for detecting odorant molecules, translating chemical signals into neural impulses that enable the sense of smell. OR4D10 is encoded by the *OR4D10* gene, located on human chromosome 17. Although its specific ligands remain largely uncharacterized, it is hypothesized to participate in odorant recognition through interactions with volatile compounds.
Structurally, OR4D10 features seven transmembrane α-helices, a hallmark of GPCRs, and is expressed on the cell surface of olfactory sensory neurons. Beyond its role in olfaction, emerging studies suggest ectopic expression of OR4D10 in non-olfactory tissues, implying potential roles in physiological or pathological processes such as cellular signaling, metabolism, or disease mechanisms.
Recombinant OR4D10 protein is produced via heterologous expression systems (e.g., bacterial, yeast, or mammalian cells) to enable functional and structural studies. These systems allow researchers to investigate ligand-binding properties, activation pathways, and downstream signaling interactions. Recombinant OR4D10 is critical for elucidating its biological significance, including its involvement in diseases or sensory mechanisms. However, challenges persist in achieving proper folding and post-translational modifications in vitro, which are essential for functional studies.
Overall, OR4D10 serves as a model for understanding olfactory receptor biology and their broader implications in human health and disease.
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