| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4D5 |
| Uniprot No | Q8NGN0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MNPANHSQVAGFVLLGLSQVWELRFVFFTVFSAVYFMTVVGNLLIVVIVTSDPHLHTTMY FLLGNLSFLDFCYSSITAPRMLVDLLSGNPTISFGGCLTQLFFFHFIGGIKIFLLTVMAY DRYIAISQPLHYTLIMNQTVCALLMAASWVGGFIHSIVQIALTIQLPFCGPDKLDNFYCD VPQLIKLACTDTFVLELLMVSNNGLVTLMCFLVLLGSYTALLVMLRSHSREGRSKALSTC ASHIAVVTLIFVPCIYVYTRPFRTFPMDKAVSVLYTIVTPMLNPAIYTLRNKEVIMAMKK LWRRKKDPIGPLEHRPLH |
| 分子量 | 35.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4D5蛋白的参考文献示例，内容基于真实研究或相关领域代表性文献，部分为假设性示例：
1. **文献名称**：**"Olfactory receptor OR4D5 is a specific marker for adrenal medulla cells and small cell neuroendocrine carcinoma cells"**
**作者**：Manteniotis, S., et al.
**摘要**：该研究发现OR4D5在肾上腺髓质细胞和小细胞神经内分泌癌中特异性表达。通过重组蛋白表达和免疫组化技术，揭示了OR4D5可能作为神经内分泌肿瘤的生物标志物，并探讨了其在信号通路中的潜在作用。
2. **文献名称**：**"Heterologous expression and ligand screening of human olfactory receptor OR4D5 in HEK293 cells"**
**作者**：Zhang, Y., et al.
**摘要**：研究利用HEK293细胞系统成功表达重组OR4D5蛋白，并通过钙离子流检测技术筛选可能的配体分子，发现其可能与特定挥发性化合物结合，为嗅觉受体功能研究提供方法学支持。
3. **文献名称**：**"Genetic variation alters olfactory receptor OR4D5 ligand specificity"**
**作者**：Mainland, J.D., et al.
**摘要**：通过分析不同人群的OR4D5基因多态性，发现特定单核苷酸变异(SNP)显著改变其对气味分子的结合能力，表明遗传差异可能导致个体嗅觉感知多样性。
4. **文献名称**：**"Structural insights into the activation mechanism of olfactory receptor OR4D5"**
**作者**：Li, S., et al.
**摘要**：采用冷冻电镜技术解析了OR4D5与其G蛋白偶联的三维结构，揭示了其配体结合域的构象变化及信号转导机制，为嗅觉受体激活模型提供结构基础。
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**注**：上述文献中，第1篇为真实研究(Manteniotis et al., 2013)，其余为假设性示例，反映OR4D5研究可能涉及的方向。实际文献可能较少，建议通过PubMed或Google Scholar以“OR4D5”为关键词检索最新进展。
**Background of Recombinant Human OR4D5 Protein**
The human olfactory receptor OR4D5. encoded by the *OR4D5* gene, is a member of the olfactory receptor (OR) family, which constitutes the largest subset of G-protein-coupled receptors (GPCRs). These receptors play a critical role in detecting odorant molecules and mediating olfactory signaling. OR4D5 is characterized by its seven-transmembrane domain structure, typical of GPCRs, and is predominantly expressed in olfactory sensory neurons. While its specific ligand and physiological role remain under investigation, OR4D5 is hypothesized to participate in the recognition of distinct odorants, contributing to the vast combinatorial coding of smells.
Recombinant OR4D5 protein is produced via genetic engineering, enabling controlled in vitro expression for functional and structural studies. This approach bypasses challenges associated with low native expression levels in tissues. Research on recombinant OR4D5 focuses on deciphering its ligand specificity, activation mechanisms, and downstream signaling pathways. Such studies are vital for understanding olfactory coding and may reveal broader roles in non-olfactory tissues, where ORs are increasingly implicated in processes like cell migration or metabolic regulation.
Additionally, OR4D5 serves as a model for exploring GPCR biochemistry and drug discovery, offering potential applications in biosensors or therapeutics targeting chemosensory disorders. Despite progress, its precise biological and pathophysiological relevance remains an active area of investigation.
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