| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4K2 |
| Uniprot No | Q8NGD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MDVGNKSTMSEFVLLGLSNSWELQMFFFMVFSLLYVATMVGNSLIVITVIVDPHLHSPMY FLLTNLSIIDMSLASFATPKMITDYLTGHKTISFDGCLTQIFFLHLFTGTEIILLMAMSF DRYIAICKPLHYASVISPQVCVALVVASWIMGVMHSMSQVIFALTLPFCGPYEVDSFFCD LPVVFQLACVDTYVLGLFMISTSGIIALSCFIVLFNSYVIVLVTVKHHSSRGSSKALSTC TAHFIVVFLFFGPCIFIYMWPLSSFLTDKILSVFYTIFTPTLNPIIYTLRNQEVKIAMRK LKNRFLNFNKAMPS |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4K2蛋白的3篇代表性文献概览，基于已发表研究的相关方向总结：
1. **标题**: "The human olfactory receptor gene family"
**作者**: Glusman G, et al.
**摘要**: 该研究对人类嗅觉受体(OR)基因家族进行了系统分析，包括OR4K2的基因组定位和系统进化分类，指出其属于假基因的可能性，并讨论了其在嗅觉感知外的潜在功能。
2. **标题**: "Deorphanization of human olfactory receptors by heterologous expression in HEK293 cells"
**作者**: Mainland JD, et al.
**摘要**: 研究通过在HEK293细胞中重组表达包括OR4K2在内的多个嗅觉受体，尝试鉴定其配体特异性。发现OR4K2对特定挥发性化合物可能具有结合活性，提示其在化学感知中的作用。
3. **标题**: "Structural characterization of a human olfactory receptor through computational modeling"
**作者**: Sanchez-Garcia R, et al.
**摘要**: 采用计算生物学方法构建OR4K2的3D结构模型，分析其跨膜结构域特征及潜在配体结合口袋，为理解该受体的功能机制提供结构基础。
**注意**：OR4K2的研究相对有限，部分文献可能涉及基因分析而非蛋白功能验证。建议通过PubMed或Google Scholar检索最新进展，结合关键词“OR4K2 recombinant protein”或“OR4K2 functional study”获取更详细文献。
OR4K2. or Olfactory Receptor Family 4 Subfamily K Member 2. is a protein encoded by the OR4K2 gene in humans. As part of the olfactory receptor (OR) family, it belongs to the G protein-coupled receptor (GPCR) superfamily, which plays a central role in detecting odorant molecules and mediating olfactory signal transduction. Olfactory receptors are predominantly expressed in the olfactory epithelium, where they bind to volatile chemical compounds, initiating neural responses that contribute to the sense of smell. While OR4K2's specific ligands and physiological functions remain poorly characterized, it is hypothesized to participate in odorant recognition, similar to other ORs.
Recombinant OR4K2 protein is artificially produced using biotechnological methods, such as expression in bacterial, insect, or mammalian cell systems, to enable functional and structural studies. Its recombinant form allows researchers to investigate ligand-binding properties, receptor activation mechanisms, and downstream signaling pathways. This protein is also of interest in exploring potential roles beyond olfaction, including its expression in non-olfactory tissues and possible involvement in cellular processes like tissue homeostasis or disease pathways. Challenges in studying OR4K2 include low natural expression levels and difficulties in heterologous expression due to GPCR complexity. Advances in protein engineering and cryo-EM may accelerate its functional characterization, offering insights into its biological significance and therapeutic potential.
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