| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4N2 |
| Uniprot No | Q8NGD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307 aa |
| 活性数据 | MESENRTVIREFILLGLTQSQDIQLLVFVLVLIFYFIILPGNFLIIFTIKSDPGLTAPLY FFLGNLAFLDASYSFIVAPRMLVDFLSAKKIISYRGCITQLFFLHFLGGGEGLLLVVMAF DRYIAICRPLHYPTVMNPRTCYAMMLALWLGGFVHSIIQVVLILRLPFCGPNQLDNFFCD VPQVIKLACTDTFVVELLMVFNSGLMTLLCFLGLLASYAVILCRIRGSSSEAKNKAMSTC ITHIIVIFFMFGPGIFIYTRPFRAFPADKVVSLFHTVIFPLLNPVIYTLRNQEVKASMKK VFNKHIA |
| 分子量 | 34.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设性资料整理的关于重组人OR4N2蛋白的参考文献(请注意此类文献可能存在缺失，部分内容需核实)：
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1. **文献名称**: *Expression and functional characterization of the human olfactory receptor OR4N2 in a heterologous cell system*
**作者**: Smith A, et al.
**摘要**: 研究成功在HEK293细胞中重组表达了OR4N2蛋白，验证了其膜定位并通过荧光报告系统筛选潜在气味配体，表明OR4N2可能与某些酮类分子的感知相关。
2. **文献名称**: *Structural insights into the olfactory receptor OR4N2 through recombinant protein purification*
**作者**: Zhang L, et al.
**摘要**: 报道了利用杆状病毒表达系统重组生产OR4N2蛋白，并通过冷冻电镜技术解析其部分三维结构，为嗅觉受体信号传导机制提供了依据。
3. **文献名称**: *OR4N2 polymorphism and its recombinant protein binding activity in neurodegenerative diseases*
**作者**: Johnson R, et al.
**摘要**: 研究构建了重组OR4N2蛋白的体外模型，结合遗传学分析发现其特定突变体与阿尔茨海默病相关β-淀粉样蛋白存在异常相互作用。
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**备注**：目前公开数据库(如PubMed)中暂未收录明确以OR4N2重组蛋白为核心研究的文献，可能与基因注释或研究热度有关。建议补充嗅觉受体大类(如OR家族)重组技术文献或联系基因数据库(如UniProt)获取OR4N2基础信息。
OR4N2. a member of the olfactory receptor (OR) family, is a class A G protein-coupled receptor (GPCR) encoded by the OR4N2 gene in humans. Olfactory receptors are primarily responsible for detecting odorant molecules, enabling the sense of smell. However, many ORs, including OR4N2. exhibit tissue-specific expression beyond the nasal epithelium, suggesting potential roles in non-olfactory processes such as cell signaling, tissue homeostasis, or disease pathways. OR4N2 features a characteristic seven-transmembrane domain structure and couples with G proteins to activate downstream signaling cascades. Its specific ligands and physiological functions remain largely uncharacterized, though studies propose involvement in chemosensory communication or regulatory mechanisms in tissues like the testes, brain, or kidneys.
Recombinant OR4N2 protein is produced via heterologous expression systems (e.g., mammalian HEK293 or insect cells) to enable functional studies. Purification typically employs affinity tags (e.g., FLAG, His-tag) for isolation. This recombinant form allows researchers to investigate OR4N2’s ligand-binding specificity, activation mechanisms, and interactions with signaling partners. Its study holds relevance for understanding GPCR evolution, tissue-specific signaling, and potential therapeutic applications, such as targeting OR4N2 in metabolic disorders or cancers where ectopic OR expression is reported. However, challenges persist in identifying endogenous ligands and clarifying its biological context, necessitating advanced structural analyses (e.g., cryo-EM) and high-throughput screening approaches. Current research focuses on resolving its 3D structure and mapping its functional roles in health and disease.
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