| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR56B4 |
| Uniprot No | Q8NH76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319 aa |
| 活性数据 | MDTSTSVTYDSSLQISQFILMGLPGIHEWQHWLSLPLTLLYLLALGANLLIIITIQHETV LHEPMYHLLGILAVVDIGLATTIMPKILAIFWFDAKAISLPMCFAQIYAIHCFFCIESGI FLCMAVDRYIAICRPLQYPSIVTKAFVFKATGFIMLRNGLLTIPVPILAAQRHYCSRNEI EHCLCSNLGVISLACDDITVNKFYQLMLAWVLVGSDMALVFSSYAVILHSVLRLNSAEAM SKALSTCSSHLILILFHTGIIVLSVTHLAEKKIPLIPVFLNVLHNVIPPALNPLACALRM HKLRLGFQRLLGLGQDVSK |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR56B4蛋白的假设性参考文献示例，基于现有嗅觉受体研究的常见方向。请注意，这些文献为模拟内容，实际文献需通过学术数据库验证：
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1. **标题**: *"Functional Expression of Human Olfactory Receptor OR56B4 in a Heterologous Cell System"*
**作者**: Zhang, Y., & Wang, L.
**摘要**: 研究了OR56B4在HEK293细胞中的重组表达，利用荧光报告系统验证其通过cAMP信号通路响应特定挥发性分子，为体外筛选配体提供方法学基础。
2. **标题**: *"Ligand Screening of OR56B4 Reveals Activation by Short-Chain Fatty Acids"*
**作者**: Müller, C., et al.
**摘要**: 通过高通量筛选鉴定OR56B4的候选配体，发现其可被丙酸等短链脂肪酸激活，提示在肠道或代谢调控中的潜在非嗅觉功能。
3. **标题**: *"Structural Characterization of OR56B4 Using Cryo-EM and Molecular Dynamics Simulations"*
**作者**: Kim, S., & Patel, R.
**摘要**: 解析了OR56B4的冷冻电镜结构，结合分子动力学模拟揭示配体结合位点特征，为嗅觉受体激活机制提供结构生物学依据。
4. **标题**: *"OR56B4 in Human Keratinocytes: A Role in Inflammatory Response"*
**作者**: Gomez, F., et al.
**摘要**: 发现OR56B4在皮肤角质细胞中表达，并通过MAPK通路介导炎症因子释放，表明其在皮肤屏障功能中的作用。
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**备注**：OR56B4的研究可能尚处早期阶段，实际文献需通过**PubMed**、**Google Scholar**等平台，以关键词“OR56B4”、“olfactory receptor 56B4”或“recombinant OR56B4”检索。若文献有限，建议扩展至嗅觉受体家族(如OR51/52/56家族)的重组表达或功能研究。
The human OR56B4 protein is a member of the olfactory receptor (OR) family, which constitutes the largest group of G protein-coupled receptors (GPCRs) in the human genome. Olfactory receptors are primarily expressed in the olfactory epithelium, where they mediate the detection of odorant molecules by binding to specific chemical structures, initiating signal transduction cascades that translate into the perception of smell. OR56B4. encoded by the OR56B4 gene located on chromosome 11. is a class II olfactory receptor, typically associated with recognizing a subset of environmental odorants.
Beyond its canonical role in olfaction, emerging studies suggest ectopic expression of OR56B4 in non-olfactory tissues, hinting at potential non-sensory functions. For instance, ORs have been implicated in processes like cell migration, apoptosis, and metabolic regulation, though OR56B4's specific physiological roles remain under investigation. Recombinant OR56B4 protein, produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), enables functional characterization, including ligand identification, receptor activation assays, and structural studies. Challenges in recombinant production include achieving proper membrane localization and functional folding, common hurdles for GPCRs. Current research aims to unravel OR56B4's ligand specificity, signaling pathways, and potential therapeutic relevance, particularly in diseases linked to chemosensory dysregulation or OR-mediated ectopic signaling.
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