| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5F1 |
| Uniprot No | O95221 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MTRKNYTSLTEFVLLGLADTLELQIILFLFFLVIYTLTVLGNLGMILLIRIDSQLHTPMY FFLANLSFVDVCNSTTITPKMLADLLSEKKTISFAGCFLQMYFFISLATTECILFGLMAY DRYAAICRPLLYSLIMSRTVYLKMAAGAFAAGLLNFMVNTSHVSSLSFCDSNVIHHFFCD SPPLFKLSCSDTILKESISSILAGVNIVGTLLVILSSYSYVLFSIFSMHSGEGRHRAFST CASHLTAIILFYATCIYTYLRPSSSYSLNQDKVASVFYTVVIPMLNPLIYSLRSKEVKKA LANVISRKRTSSFL |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5F1蛋白的模拟参考文献示例（注：以下内容为虚构生成，用于示例格式）：
1. **文献名称**：*Structural and functional characterization of recombinant human OR5F1 olfactory receptor*
**作者**：Smith, J.R., et al.
**摘要**：本研究通过哺乳动物表达系统成功重组表达了OR5F1蛋白，解析了其三维结构，并通过钙离子流实验鉴定了其与特定醛类化合物的结合活性，揭示了其在嗅觉信号传导中的作用机制。
2. **文献名称**：*OR5F1 expression in non-olfactory tissues: Implications for cancer biomarker discovery*
**作者**：Li, X., & Wang, H.
**摘要**：首次报道了OR5F1在结肠癌组织中的异常表达，重组蛋白实验表明其可能通过调控MAPK信号通路影响癌细胞迁移，为癌症诊断提供了潜在靶点。
3. **文献名称**：*Ligand specificity mapping of OR5F1 using a yeast surface display platform*
**作者**：Kim, S., et al.
**摘要**：利用酵母表面展示技术重组表达OR5F1，筛选了200种气味分子，发现其对庚醛和辛醛具有高亲和力，为嗅觉受体配体选择性的进化研究提供了数据支持。
4. **文献名称**：*G protein coupling mechanisms of OR5F1 revealed by cryo-EM*
**作者**：Zhang, Y., et al.
**摘要**：通过冷冻电镜技术解析了OR5F1与Gαs蛋白偶联的复合体结构，阐明了其激活下游cAMP信号通路的构象变化，为嗅觉受体药物开发提供结构基础。
（注：实际研究中OR5F1相关文献可能较少，建议通过PubMed或Google Scholar以“OR5F1”、“recombinant OR5F1”等关键词检索最新成果。）
Olfactory receptor 5F1 (OR5F1) is a member of the olfactory receptor (OR) family, a large group of G protein-coupled receptors (GPCRs) primarily involved in odorant detection. Located on chromosome 11 in humans, OR5F1 is classified as a class II olfactory receptor, typically expressed in the olfactory epithelium. Though its specific ligand remains uncharacterized, it is hypothesized to bind volatile odorant molecules, triggering intracellular signaling cascades via cAMP pathways. Like many olfactory receptors, OR5F1 exhibits limited tissue specificity, though emerging studies suggest potential ectopic expression in non-olfactory tissues, hinting at broader physiological roles beyond smell.
Recombinant OR5F1 protein is synthesized using heterologous expression systems (e.g., mammalian or insect cells) to study its structure-function relationships, ligand specificity, and signaling mechanisms. Challenges in producing functional OR proteins—due to poor solubility and membrane localization requirements—often necessitate fusion tags or specialized vectors. Research on recombinant OR5F1 contributes to decoding the combinatorial coding of olfaction and may aid in developing biosensors or therapeutic strategies targeting OR-associated pathologies, such as anosmia or metabolic disorders. Its structural characterization also provides insights into GPCR evolution and diversity. However, functional studies remain preliminary, underscoring the need for advanced cellular assays and ligand-screening platforms.
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