| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5B3 |
| Uniprot No | Q8NH48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MENKTEVTQFILLGLTNDSELQVPLFITFPFIYIITLVGNLGIIVLIFWDSCLHNPMYFF LSNLSLVDFCYSSAVTPIVMAGFLIEDKVISYNACAAQMYIFVAFATVENYLLASMAYDR YAAVCKPLHYTTTMTTTVCARLAIGSYLCGFLNASIHTGDTFSLSFCKSNEVHHFFCDIP AVMVLSCSDRHISELVLIYVVSFNIFIALLVILISYTFIFITILKMHSASVYQKPLSTCA SHFIAVGIFYGTIIFMYLQPSSSHSMDTDKMAPVFYTMVIPMLNPLVYSLRNKEVKSAFK KVVEKAKLSVGWSV |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性参考文献的示例(请注意，OR5B3相关研究文献有限，实际引用需通过数据库验证)：
1. **"Functional characterization of recombinant human olfactory receptor OR5B3 expressed in HEK293 cells"**
*Authors: Smith A, et al. (2018)*
摘要：利用HEK293细胞成功表达OR5B3蛋白，通过钙离子信号检测发现其对特定醛类气味分子(如癸醛)具有选择性响应，证实其配体结合特性。
2. **"Structural insights into OR5B3 receptor activation using cryo-EM"**
*Authors: Zhang Y, et al. (2020)*
摘要：首次解析OR5B3与激动剂结合的冷冻电镜结构，揭示其跨膜结构域构象变化及G蛋白偶联机制，为嗅觉受体激活模型提供结构依据。
3. **"OR5B3 expression in non-olfactory tissues: Implications in prostate cancer signaling"**
*Authors: Johnson R, et al. (2019)*
摘要：发现OR5B3在前列腺癌细胞中异常表达，重组蛋白实验表明其通过MAPK通路调控癌细胞迁移，提示潜在肿瘤标志物功能。
4. **"Optimization of recombinant OR5B3 production in insect cells for high-throughput screening"**
*Authors: Lee S, et al. (2021)*
摘要：开发杆状病毒-昆虫细胞表达系统，通过密码子优化和膜蛋白纯化策略提升OR5B3产量，用于大规模配体筛选平台构建。
**建议**：实际研究中请通过PubMed、Web of Science等平台以 **"OR5B3"、"olfactory receptor 5B3"、"OR5B3 recombinant"** 为关键词检索最新文献，部分研究可能涉及嗅觉机制、异源表达优化或疾病关联方向。
**Background of Recombinant Human OR5B3 Protein**
The recombinant human OR5B3 protein is a synthesized form of the olfactory receptor family 5 subfamily B member 3. a G protein-coupled receptor (GPCR) primarily associated with olfactory signal transduction. Olfactory receptors (ORs) are crucial for detecting odorant molecules, initiating neural responses that enable smell perception. OR5B3. encoded by the *OR5B3* gene, is expressed in olfactory epithelial tissues, though extra-nasal expression has been observed in certain tissues, suggesting potential non-olfactory roles, such as in cellular signaling or disease pathways.
As a recombinant protein, OR5B3 is produced in heterologous systems (e.g., bacterial, yeast, or mammalian cells) to enable functional studies. Its recombinant form often includes tags (e.g., His-tag) for purification and detection. Research focuses on characterizing its ligand specificity, activation mechanisms, and downstream signaling, which may provide insights into odorant-receptor interactions or therapeutic targets. OR5B3 has also been studied in contexts beyond olfaction, including its potential involvement in diseases like cancer, where abnormal OR expression is increasingly noted.
Despite challenges in expressing functional GPCRs *in vitro*, advances in protein engineering have facilitated structural and pharmacological studies of OR5B3. contributing to the broader understanding of olfactory receptor biology and their applications in biosensors or drug discovery.
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