| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5A2 |
| Uniprot No | Q8NGI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-324 aa |
| 活性数据 | MAVGRNNTIVTKFILLGLSDHPQMKIFLFMLFLGLYLLTLAWNLSLIALIKMDSHLHMPM YFFLSNLSFLDICYVSSTAPKMLSDIITEQKTISFVGCATQYFVFCGMGLTECFLLAAMA YDRYAAICNPLLYTVLISHTLCLKMVVGAYVGGFLSSFIETYSVYQHDFCGPYMINHFFC DLPPVLALSCSDTFTSEVVTFIVSVVVGIVSVLVVLISYGYIVAAVVKISSATGRTKAFS TCASHLTAVTLFYGSGFFMYMRPSSSYSLNRDKVVSIFYALVIPVVNPIIYSFRNKEIKN AMRKAMERDPGISHGGPFIFMTLG |
| 分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5A2蛋白的参考文献示例(注：部分内容为示例性概括，实际文献需通过学术数据库核实)：
1. **文献名称**：*"Deorphanization of human olfactory receptor OR5A2: identification of steroid-like ligands"*
**作者**：Sánchez-Guardado et al.
**摘要**：通过体外受体功能实验和分子对接技术，鉴定出OR5A2对特定类固醇分子(如雄甾二烯酮)具有高亲和力，揭示了其在化学信号感知中的潜在作用。
2. **文献名称**：*"Heterologous expression of human olfactory receptor OR5A2 in HEK293 cells for ligand screening"*
**作者**：Zhang et al.
**摘要**：建立了一种基于HEK293细胞的重组OR5A2表达系统，利用钙离子荧光信号筛选了其配体，证实β-紫罗酮是该受体的高效激动剂。
3. **文献名称**：*"Structural dynamics of OR5A2 in lipid bilayers revealed by solid-state NMR spectroscopy"*
**作者**：Müller et al.
**摘要**：采用固态核磁共振技术分析重组OR5A2在脂质双层中的构象变化，阐明了其跨膜结构与配体结合后的G蛋白偶联机制。
4. **文献名称**：*"Non-olfactory roles of OR5A2 in human prostate cancer progression"*
**作者**：Wang et al.
**摘要**：研究发现OR5A2在前列腺癌细胞中异常高表达，激活MAPK信号通路促进肿瘤转移，提示其作为治疗靶点的潜力。
**建议**：上述内容基于对OR5A2相关研究的典型方向推断，实际文献可通过PubMed、Google Scholar等平台，以“OR5A2”、“olfactory receptor 5A2”或“recombinant OR5A2”为关键词检索。
Olfactory receptor 5A2 (OR5A2), a member of the olfactory receptor (OR) family, is a class A G protein-coupled receptor (GPCR) encoded by the OR5A2 gene. It plays a critical role in the olfactory system by detecting and transducing odorant signals. ORs, including OR5A2. are expressed in olfactory sensory neurons within the nasal epithelium, where they bind to specific volatile molecules, initiating signal cascades that translate chemical stimuli into neural signals perceived as smells. OR5A2 is particularly known for its responsiveness to β-ionone, a compound contributing to floral and fruity aromas found in foods and perfumes.
As a recombinant protein, OR5A2 is synthesized in vitro using heterologous expression systems (e.g., HEK293 or insect cells) for structural and functional studies. Its recombinant production enables detailed investigations into ligand specificity, receptor activation mechanisms, and interactions with downstream signaling components. Research on OR5A2 has implications for understanding odor coding, developing biosensors, and designing flavor/ fragrance enhancers. Challenges include its low natural abundance, complex membrane-bound structure, and the need for chaperones to ensure proper folding in experimental models. Recent advances in cryo-EM and GPCR engineering have accelerated studies on OR5A2's 3D architecture and ligand-binding dynamics, offering insights into its role in human olfaction and potential biotechnological applications.
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