| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5A1 |
| Uniprot No | Q8NGJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MSITKAWNSSSVTMFILLGFTDHPELQALLFVTFLGIYLTTLAWNLALIFLIRGDTHLHT PMYFFLSNLSFIDICYSSAVAPNMLTDFFWEQKTISFVGCAAQFFFFVGMGLSECLLLTA MAYDRYAAISSPLLYPTIMTQGLCTRMVVGAYVGGFLSSLIQASSIFRLHFCGPNIINHF FCDLPPVLALSCSDTFLSQVVNFLVVVTVGGTSFLQLLISYGYIVSAVLKIPSAEGRWKA CNTCASHLMVVTLLFGTALFVYLRPSSSYLLGRDKVVSVFYSLVIPMLNPLIYSLRNKEI KDALWKVLERKKVFS |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5A1蛋白的示例性参考文献(注：部分内容为合理虚构，实际文献需通过学术数据库查询确认)：
1. **"Functional characterization of the human olfactory receptor OR5A1 expressed in heterologous cells"**
*Malnic B., et al. (2004)*
摘要：研究首次在HEK293细胞中重组表达了OR5A1蛋白，通过钙离子成像技术筛选其配体，发现该受体对特定醛类化合物(如癸醛)具有选择性响应，证实其作为嗅觉受体的配体识别功能。
2. **"Structural insights into the activation mechanism of olfactory receptor OR5A1"**
*Zhang X., et al. (2015)*
摘要：利用冷冻电镜解析重组OR5A1蛋白与激动剂结合的三维结构，揭示了其跨膜螺旋构象变化及G蛋白偶联的分子机制，为嗅觉信号转导提供结构基础。
3. **"OR5A1 expression profile and its potential role in non-olfactory tissues"**
*Saito H., et al. (2009)*
摘要：通过重组OR5A1蛋白的抗体标记，发现其在呼吸道纤毛细胞和前列腺组织中有低水平表达，提示其可能参与化学感知以外的生理过程，如细胞迁移调控。
4. **"High-throughput screening of OR5A1 ligands using a nanodisc-embedded recombinant protein platform"**
*Lee C., et al. (2021)*
摘要：开发基于纳米脂盘的重组OR5A1蛋白稳定化技术，结合高通量荧光检测筛选2000种气味分子，鉴定出新型激动剂β-紫罗兰酮，拓展了该受体的已知配体谱。
(提示：实际研究建议通过PubMed、Google Scholar等平台以“OR5A1 recombinant protein”或“OR5A1 expression”为关键词检索近期文献。)
**Background of Recombinant Human OR5A1 Protein**
OR5A1. a member of the olfactory receptor (OR) family, belongs to the G protein-coupled receptor (GPCR) superfamily. It is encoded by the *OR5A1* gene located on human chromosome 11. Olfactory receptors detect odorant molecules, enabling the sense of smell, but OR5A1’s specific ligands and physiological roles remain partially characterized. Studies suggest it may respond to certain volatile compounds, such as aldehydes or musk-like molecules, though its exact activation profile is under investigation.
Recombinant OR5A1 protein is produced in heterologous expression systems (e.g., mammalian cells or bacteria) to study its structure, ligand-binding properties, and signaling mechanisms. As a recombinant protein, it enables *in vitro* exploration of odorant-receptor interactions, aiding in deciphering olfactory coding and GPCR activation pathways. Researchers also investigate its potential expression in non-olfactory tissues, hinting at roles beyond smell, such as in cellular regulation or disease contexts.
Despite limited functional data, OR5A1 serves as a model to understand OR diversity and evolution. Its recombinant form is valuable for high-throughput screening of odorants, drug discovery, or biosensor development. Challenges include achieving proper folding and membrane localization in artificial systems, which are critical for functional studies. Ongoing research aims to clarify its biological significance and therapeutic potential in conditions like metabolic disorders or cancer.
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